Abstract
We have already reported that exercise activates kynurenine pathway. But, the mechanism for this activation by exercise is still unclear. Kynurenine is metabolized to NAD, which is an essential factor for energy metabolism. In this study, exercise on treadmill was loaded to rats until all-out and tryptophan metabolites and tryptophan 2,3dioxygenase (TDO) and indoleamine 2,3-dioxygenase (IDO) activities were determined in the blood, livers, and macrophages, respectively, in the exercise-loaded rats. The mean values of serum tryptophan concentration decreased from 92.6/6.0 nmol/ml to 52.4/10.2 nmol/ml (p<0.05) just after treadmill load. The serum kynurenine concentration had increased from 2.0610.25 nmol/ml to 3.0810.62 nmoUml (p<0.005). And whole blood NAD concentration increased from 68.8±14.6 nmol/ml to 77.9119.1 nmol/ml (p<0.005). These results showed that exercise activated the kynurenine pathway of tryptophan metabolism and made NAD which will be concerned with energy metabolism in mitochondria. Tryptophan-NAD pathway was initiated by cleavage of indole ring of tryptophan by TDO in the liver and IDO in many organs. We had also found that the exercise increase IDO activity of macrophages, but not TDO activity.
Keywords
- Quinolinic Acid
- Kynurenine Pathway
- Tryptophan Metabolism
- Intensive Exercise
- Tryptophan Metabolite
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References
D.A. Bender, Biochemistry of tryptophan in health and diseaseMol. Aspects Med.6, 101–97 (1983).
Y. Ozeki, M.P. Edelstein, and D.S. Duch, Induction of indoleamine 2,3-dioxygenase: a mechanism of the antitumor activity of interferon gammaProc. Natl. Acad. Sci. U.S.A.85, 1242–1246 (1988).
D.H. Munn, M. Zhou, J.T. Attwood, I. Bondarev, S.J. Conway, B. Marshall, C. Brown, and A.L. Mellor, Prevention of allogeneic fetal rejection by tryptophan catabolismScience281, 1191–1193 (1998).
K. Saito, J.S. Crowley, S.P. Markey, and M.P. Heyes, A mechanism for increased quinolinic acid formation following acute systemic immune stimulationJ. Biol. Chem.268, 15496–15503 (1993).
Y. Ito, K. Maruta, Y. Nakagami, T. Koike, Y. Oguri, Y. Nagamura, and K. Saito, Kynurenine concentration of serum was increased by exerciseAdv. Exp. Med. Biol.467, 717–722 (1999).
R. Yonekura, Y. Ito, K. Shibata, T. Fukuwatari, K. Saito, Y. Oguri, K. Asayama, and Y. Nagamura, The influence of the exercise load on the tryptophan-NAD pathway in humans from running [in Japanese].J. Creative Approach Health1, 57–64 (2002).
K. Shibata, and K. Tanaka, Simple measurement of blood NADP and blood levels of NAD and NADP in humansAgric. Biol. Chem.50,2941–2942 (1986).
T. Fukuwatari, K. Shibata, K. lshihara, T. Fushiki, and E. Sugimoto, Elevation of Blood NAD Level after Moderate Exercise in Young Women and MiceJ. Nutr. Sci. Vitaminol.47, 177–179 (2001).
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Ito, Y. et al. (2003). Tryptophan Metabolism was Accelerated by Exercise in Rat. In: Allegri, G., Costa, C.V.L., Ragazzi, E., Steinhart, H., Varesio, L. (eds) Developments in Tryptophan and Serotonin Metabolism. Advances in Experimental Medicine and Biology, vol 527. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-0135-0_61
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DOI: https://doi.org/10.1007/978-1-4615-0135-0_61
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