Taurine 5 pp 349-356 | Cite as

Taurine Chloramine Inhibits Production of Inflammatory Mediators and iNOS Gene Expression in Alveolar Macrophages; a Tale of Two Pathways: Part II, IFN-γ Signaling Through JAK/Stat

  • Michael R. Quinn
  • Madhabi Barua
  • Yong Liu
  • Valeria Serban
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 526)


Taurine protects against tissue damage in a variety of models that share inflammation as a common pathogenic feature1. This has been particularly well documented in animal models of lung injury where prophylactic administration of taurine protects lung from damage induced by inhalation exposure to NO2 2 or 03 3 and by intratracheal instillation of bleomycin4,5, amidarone6, or paraquat7. Lung damage in these models is associated with an influx of neutrophils along with mononuclear leukocytes and production of inflammatory mediators, which contribute to the ensuant pulmonary damage.


Alveolar Macrophage Electrophoretic Mobility Shift Assay Nuclear Protein Extract NR8383 Cell iNOS Gene Expression 


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  1. 1.
    Quinn, M.R., and Schuller-Levis,G.B., 1999, Taurine Chloramine, an Inhibitor of iNOS Expression and a Potential Modulator of Inflammation. In Molecular and Cellular Biology of Nitric Oxide (J. Laskin and D. Laskin, eds.), Marcel Dekker, Inc., New York, pp. 309–331.Google Scholar
  2. 2.
    Gordon, R.E., Shaked, A.A., and Solano, D.F., 1986, Taurine protects hamster bronchioles from acute NO2 induced alterations. Am. J. Pathol. 125: 585–600.PubMedGoogle Scholar
  3. 3.
    Schuller-Levis, G.B., Gordon, R.E., Park, E., Pendino, K.J., and Laskin, D.L., 1995, Taurine protects rat bronchioles from acute ozone-induced lung inflammation and hyperplasia. Exp. Lung Res. 21: 877–888.PubMedCrossRefGoogle Scholar
  4. 4.
    Gurujeyalakshmi, G., Wang, Y, and Giri, S.N., 2000, Taurine and niacin block lung injury and fibrosis by down-regulating bleomycin-induced activation of transcription nuclear factor-kappa B in mice. J. Pharmacol. Exp. Ther. 293: 82–90.PubMedGoogle Scholar
  5. 5.
    Gurujeyalakshmi, G., Wang, Y, and Giri, S.N., 2000, Suppression of bleomycininduced nitric oxide production in mice by taurine and niacin. Nitric Oxide 4: 399–411.PubMedCrossRefGoogle Scholar
  6. 6.
    Wang, Q., Hollinger, M.A., and Giri, S.N., 1992, Attenuation of amiodarone induced lung fibrosis and phospholipidosis in hamsters by taurine and/or niacin treatment. J. Pharmacol. Exp. Ther. 262: 127–132.PubMedGoogle Scholar
  7. 7.
    Izmi, K., Nagata, R., Motoya, T., Yamashita, Y., Hirokane, T., Naguta, T., Satoh, Y., Sawada, Y., Ishibashi, M., Yoshida, H., and Fukuda, T., 1989, Preventive effect of taurine against acute paraquat intoxication in beagles. Jpn. J. Pharmacol. 50: 229–233.CrossRefGoogle Scholar
  8. 8.
    Zgicznski, J.M., Stelmaszynska, T., Domanski, J., and Ostrowski, W., 1971, Chloramines as intermediates of oxidation reaction of amino acids by myeloperoxidase. Biochim. Biophys. Acta 235: 419–424.CrossRefGoogle Scholar
  9. 9.
    Weiss, S.J.R., Klein, A., Slivka, A., and Wei, M., 1982, Chlorination of taurine by human neutrophils: evidence for hypochlorous acid generation. J. Clin. Invest. 70: 598–607.PubMedCrossRefGoogle Scholar
  10. 10.
    Grisham, M.B., Jefferson, M.M., Melon, D.F., and Thomas, E.L., 1984, Chlorination of endogenous amines by isolated neutrophils. J. Biol. Chem. 259: 10404–10413.PubMedGoogle Scholar
  11. 11.
    Marcinkiewicz, J., Grabowska, A., Bereta, J., and Stelmaszynska, T., 1995, Taurine chloramine, a product of activated neutrophils, inhibits in vitro the generation of nitric oxide and other macrophage inflammatory mediators. J. Leukoc. Biol. 58: 667–674.PubMedGoogle Scholar
  12. 12.
    Park, E., Alberti, J., Quinn, M.R., and Schuller-levis, G., 1998, Taurine chloramine inhibits the production of superoxide anion, IL-6 and IL-8 in activated human polymorphonuclear leukocytes. Adv. Exp. Med. Biol. 442: 177–182.PubMedGoogle Scholar
  13. 13.
    Liu, Y., Tonna-DeMasi, M., Park, E., Schuller-Levis, G., and Quinn, M.R., 1998, Taurine chloramine inhibits production of nitric oxide and prostaglandin Ez in activated C6 glioma cells by suppressing inducible nitric oxide synthase and cyclooxygenase-2 expression. Molec. Brain Res. 59: 189–195.PubMedCrossRefGoogle Scholar
  14. 14.
    Kontny, E., Grabowska, A., Kowalczewski, J., Kurowska, M., Janicka, I., Marcinkiewicz, J., and Maslinski, W., 1999, Taurine chloramine inhibition of cell proliferation and cytokine production by rheumatoid arthritis fibroblast-like synoviocytes. Arthritis Rheum. 42: 2552–2560.PubMedCrossRefGoogle Scholar
  15. 15.
    Sakanashi, Y., Takeya, M., Yoshimura, T., Feng, L., Morioka, T., and Takahashi, K., 1994, Kinetics of macrophage subpopulations and expression of monocyte chemoattractant protein-1 (MCP-1) in bleomycin-induced lung injury of rats studied by a novel monoclonal antibody against rat MCP-1. J. Leukoc. Biol. 56: 741–750.PubMedGoogle Scholar
  16. 16.
    Lentsch, A.B., Czermak, B.J., Bless, N.M., Rooijen, N.V., and Ward, PA., 1999, Essential role of alveolar macrophages in intrapulmonary activation of NF-KB. Am. J. Respir. Cell Mol. Biol. 20: 692–698.PubMedGoogle Scholar
  17. 17.
    Barua, M., Liu, Y., and Quinn, M.R., 2001, Taurine chloramine inhibits nitric oxide synthase and TNF-a gene expression in activated alveolar macrophages: Decreased NF-KB activation and IKB kinase activity. J. Immunol. 167: 2275–2281.PubMedGoogle Scholar
  18. 18.
    Liu, Y., and Quinn, M.R., 2002, Chemokine production by rat alveolar macrophages is inhibited by taurine chloramine. Immunol. Lett. 80: 27–32.PubMedCrossRefGoogle Scholar
  19. 19.
    Eberhardt, W., Kunz, D., Hummel, R., and Pfeilschifter, J., 1996, Molecular cloning of the rat inducible nitric oxide synthase gene promoter. Biochem. Biophys. Res. Commun. 223: 752–756.PubMedCrossRefGoogle Scholar
  20. 20.
    Zhang, H., Chen, X., Teng, X., Snead, C., and Catravas, J.D., 1998, Molecular cloning and analysis of the rat inducible nitric oxide synthase gene promoter in aortic smooth muscle cells. Biochem. Pharmacol. 55: 1873–1880.PubMedCrossRefGoogle Scholar
  21. 21.
    Murphy, W.J., 1999, Transcriptional Regulation of the Genes Encoding Nitric Oxide Synthase. In Cellular and Molecular Biology of Nitric Oxide (J.D. Laskin and D.L. Laskin, eds.), Marcel Dekker, Inc., New York, pp. 1–56.Google Scholar
  22. 22.
    Helmke, R.J., German, V.F., and Mangos, J.A., 1989, A continuous alveolar macrophage cell line: comparisons with freshly derived alveolar macrophages. In Vitro Cell. Develop. Biol. 25: 44–48.CrossRefGoogle Scholar
  23. 23.
    Griscavage, J.M., Rogers, N.E., Sherman, M.P., and Ignarro, L.J., 1993, Inducible nitric oxide synthase from a rat alveolar macrophage cell line is inhibited by nitric oxide. J. Immunol. 151: 6329–6337.PubMedGoogle Scholar
  24. 24.
    Martin, E., Nathan, C., and Xie, Q., 1994, Role of interferon regulatory factor 1 in induction of nitric oxide synthase. J. Exp. Med. 180: 977–984.PubMedCrossRefGoogle Scholar
  25. 25.
    Gao, J., Morrison, D.C., Parmely, T.J., Russell, S.W., and Murphy, W.J., 1997, An interferon-y-activated site (GAS) is necessary for full expression of the mouse iNOS gene in response to interferon-y and lipopolysaccharide. J. Biol. Chem. 272: 1226–1230.PubMedCrossRefGoogle Scholar
  26. 26.
    Der, S.D., Zhou, A., Williams, B.R.G., and Silverman, R.H., 1998, Identification of genes differentially regulated by interferon a, ß or y using oligonucleotide arrays. Proc. Nad. Acad. Sci. USA 95: 15623–15628.CrossRefGoogle Scholar
  27. 27.
    O’Shea, J., Gadina, M., and Schreiber, R.D., 2002, Cytokine signaling in 2002: New surprises in the Jak/Stat pathway. Cell 109: S121–S131.PubMedCrossRefGoogle Scholar
  28. 28.
    Ramana, C.V., Gil, M.P., Schreiber, R.D., and Stark, R.D., 2002, Statl-dependent and-independent pathways in IFN-y-dependent signaling. Trends Immunol. 23: 96–101.PubMedCrossRefGoogle Scholar
  29. 29.
    Starr, R., and Hilton, D.J., 1999, Negative regulation of the JAK/STAT pathway. BioEssays 21: 47–52.PubMedCrossRefGoogle Scholar
  30. 30.
    Liu, B., Liao, J., Rao, X., Kushner, S.A., Chung, C.D., Chang, D.D., and Shuai, K., 1998, Inhibition of Stat 1-mediated gene activation by PIAS1. Proc. Natl. Acad. Sci. USA 95: 10626–10631.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2003

Authors and Affiliations

  • Michael R. Quinn
    • 1
  • Madhabi Barua
    • 2
  • Yong Liu
    • 2
  • Valeria Serban
    • 1
  1. 1.Center for Developmental NeuroscienceStaten IslandUSA
  2. 2.Laboratory of Molecular Cell Signaling, Department of Developmental BiochemistryNew York State Institute for Basic Research in Developmental DisabilitiesUSA

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