Relationship Between the Gene Expression of C-FOS and Degree of Hypoxia in Rat Brain, as Revealed by Near-Infrared Spectroscopy

  • Yasutomo Nomura
  • Masataka Kinjo
  • Mamoru Tamura
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 530)


Hypoxic induction of c-fos was studied in rat brains as a function of the cerebral oxygenation state using near-infrared spectroscopy by which the hemoglobin oxygenation state and redox state of mitochondrial cytochrome oxidase can be monitored noninvasively. Following reoxygenation after hypoxia, the expression of c-fos and MAP2 mRNAs was determined by reverse transcription-coupled PCR. The expression of MAP2 remained unchanged throughout all conditions from 21 to 8% FiO2. Under the mildly hypoxic conditions, c-fos mRNA was not induced. Hemoglobin was partially deoxygenated but cytochrome oxidase remained fully oxidized. Severe hypoxia, where cytochrome oxidase was reduced, caused a significant induction of c-fos mRNA. At this stage, the oxygen concentration in cerebral tissue fell to lower than 10-7 M. These data suggest that the decline in oxidative phosphorylation might be a trigger for the induction of c-fos mRNA.

Key words

c-fos mRNA cytochrome oxidase hemoglobin hypoxia immediate early genes near infrared spectroscopy 


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Copyright information

© Springer Science+Business Media New York 2003

Authors and Affiliations

  • Yasutomo Nomura
    • 1
  • Masataka Kinjo
    • 2
  • Mamoru Tamura
    • 2
  1. 1.Deptpartment of Bio-System Engineering, Faculty of EngineeringYamagata UniversityYonezawaJapan
  2. 2.Laboratory of supramolecular Biophysics, Research Institute for Electronic ScienceHokkaido UniversitySapporoJapan

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