Abstract
Hypoxic induction of c-fos was studied in rat brains as a function of the cerebral oxygenation state using near-infrared spectroscopy by which the hemoglobin oxygenation state and redox state of mitochondrial cytochrome oxidase can be monitored noninvasively. Following reoxygenation after hypoxia, the expression of c-fos and MAP2 mRNAs was determined by reverse transcription-coupled PCR. The expression of MAP2 remained unchanged throughout all conditions from 21 to 8% FiO2. Under the mildly hypoxic conditions, c-fos mRNA was not induced. Hemoglobin was partially deoxygenated but cytochrome oxidase remained fully oxidized. Severe hypoxia, where cytochrome oxidase was reduced, caused a significant induction of c-fos mRNA. At this stage, the oxygen concentration in cerebral tissue fell to lower than 10-7 M. These data suggest that the decline in oxidative phosphorylation might be a trigger for the induction of c-fos mRNA.
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Nomura, Y., Kinjo, M., Tamura, M. (2003). Relationship Between the Gene Expression of C-FOS and Degree of Hypoxia in Rat Brain, as Revealed by Near-Infrared Spectroscopy. In: Dunn, J.F., Swartz, H.M. (eds) Oxygen Transport to Tissue XXIV. Advances in Experimental Medicine and Biology, vol 530. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-0075-9_61
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DOI: https://doi.org/10.1007/978-1-4615-0075-9_61
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