Effect of Mild Hyperglycemia ± Meta-Iodo-Benzylguanidine on the Radiation Response of R3230 Ac Tumors

  • Intae Lee
  • Jerry D. Glickson
  • Mark W. Dewhirst
  • Dennis B. Leeper
  • Randy Burd
  • Harish Poptani
  • Lydie Nadal
  • W. Gillies McKenna
  • John E. Biaglow
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 530)

Abstract

The effects of glucose or meta-iodo-benzylguanidine (MIBG) on oxygen utilization (QO2) of several tumor cell lines were studied using a Clark-type electrode chamber. For in vivo studies, rats bearing R3230 Ac rat mammary adenocarcinomas were utilized. To evaluate changes in tumor oxygenation induced by glucose or MIBG, intratumoral pO2 and skeletal muscle pO2 were measured using Eppendorf Histography. To find the effect of mild hyperglycemia (i.p., 1 g/kg) ± MIBG (i.p., 20 mg/kg) on the radiation response, a growth delay assay was used. Glucose alone produced a ~20% inhibition of QO2 in several tumor cells we tested except Q7 tumor cells. MIBG inhibited QO2 in R3230 Ac tumors. The median tumor pO2 for glucose + MIBG was increased from 5.3 mm Hg to 13.8 mm Hg. We hypothesized that combined treatment with glucose + MIBG significantly enhanced radiation-induced tumoricidal effects on R3230 Ac tumors, mainly due to reduction in QO2 and increase in tumor pO2.

Key words

glucose meta-iodo-benzylguanidine QO2 pO2 radiation response 

Abbreviations

MIBG

meta-iodo-benzylguanidine

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Copyright information

© Springer Science+Business Media New York 2003

Authors and Affiliations

  • Intae Lee
    • 1
  • Jerry D. Glickson
    • 2
  • Mark W. Dewhirst
    • 3
  • Dennis B. Leeper
    • 4
  • Randy Burd
    • 4
  • Harish Poptani
    • 2
  • Lydie Nadal
    • 2
  • W. Gillies McKenna
    • 1
  • John E. Biaglow
    • 1
  1. 1.Department of Radiation OncologyUniversity of Pennsylvania School of MedicinePhiladelphiaUSA
  2. 2.Department of RadiologyUniversity of Pennsylvania School of MedicinePhiladelphiaUSA
  3. 3.Department of Radiation OncologyDuke University Medical CenterDurhamUSA
  4. 4.Department of Radiation OncologyThomas Jefferson UniversityPhiladelphiaUSA

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