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Streptozotocin-Induced Diabetes — A Rat Model to Study Involvement of Retinal Cell Types in the Onset of Diabetic Retinopathy

  • Elisabeth Rungger-Brändle
  • André A. Dosso
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 533)

Abstract

Diabetic retinopathy (DR) is a potentially blinding complication of diabetes mellitus. After the initial diagnosis of diabetes, the deficiencies typical for DR, namely abnormal autoregulation of retinal arteries, increased blood flow, breakdown of the blood-retinal barrier, and disturbances in retinal function develop silently and remain clinically undetected for quite some time. A characteristic microangiopathy in the fundus is one of the first visible signs of DR. Macular edema, neovascularization, contraction of the vitreous and of proliferated fibrovascular tissue are late consequences in the diabetic eye. The severity of these lesions is closely related to the duration of diabetes, to blood glucose control, and to risk factors such as hypertension.

Keywords

Vascular Endothelial Growth Factor Diabetic Retinopathy Retinal Pigment Epithelium Proliferative Diabetic Retinopathy Evans Blue 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Adamis, A.P., 2002, Is diabetic retinopathy an inflammatory disease?Br. J. Ophthalmol 86:363.PubMedCrossRefGoogle Scholar
  2. Ambati, J., Chalam, K., Chawla, D., D’Angio, C., Guillet, E., Rose, S., Vanderlinde, R., and Ambati, B., 1997, Elevated gamma-aminobutyric acid, glutamate, and vascular endothelial growth factor levels in the vitreous of patients with proliferative diabetic retinopathy.Arch. Ophthalmol. 115:1161.PubMedCrossRefGoogle Scholar
  3. Barouch, F.C., Miyamoto, K., Allprot, J.R., Fujita, K., Bursell, S.-E., Aiello, L.P., Luscinskas, F.W., and Adamis, A.P., 2000, Integrin-mediated neutrophil adhesion and retinal leukostasis in diabetes.Invest. Ophthalmol Vis. Sci. 41:1153.PubMedGoogle Scholar
  4. Bates, D.O., Hillman, N.J., Williams, B., Neal, C.R., and Pocock, T.M., 2002, Regulation of microvascular permeability by vascular endothelial growth factors.J. Anat. 200:581.PubMedCrossRefGoogle Scholar
  5. Bok, D., Senanayake, P., Calabro, A., Hu, J.G., and Hollyfield, J.G., 2002, Glycogen synthesis in human RPE. Invest. Ophthalmol. Vis. Sci. ARVO no. 4569 43:182.Google Scholar
  6. Crooks, J., and Kolb, H., 1992, Localization of GABA, glycine, glutamate and tyrosine hydroxylase in the human retina.J. comp. Neurol. 315:287.PubMedCrossRefGoogle Scholar
  7. Do Carmo, A., Ramos, P., Reis, A., Proença, R., and Cunha-Vaz, J.G., 1998, Breakdown of the inner and outer blood retinal barrier in streptozotocin-induced diabetes.Exp. Eye Res.67: 569.CrossRefGoogle Scholar
  8. Dosso, A.A., Bonvin, E.R., Morel, Y., Golay, A., Assal, J.P., and Leuenberger, P.M., 1996, Risk factors with contrast sensitivity loss in diabetic patients.Graefes Arch. Clin. Exp. Ophthalmol. 234:300.PubMedCrossRefGoogle Scholar
  9. Hardy, K.J., Lipton, J., Foster, D.H., and Scarpello, J.H.B., 1992, Detection of colour vision abnormalities in uncomplicated type I diabetic patients with angiographically normal retinas.Br. J. Ophthalmol.76: 461.PubMedCrossRefGoogle Scholar
  10. Ishikawa, A., Ishiguro, S., and Tamai, M., 1996, Changes in GABA metabolism in streptozotocin-induced diabetic rat retinas.Curr. Eye Res. 15:63.PubMedCrossRefGoogle Scholar
  11. Kalloniatis, M., and Napper, G.A., 2002, Retinal neurochemical changes following application of glutamate as a metabolic substrate.Clin. Exp. Optom. 85:27.PubMedCrossRefGoogle Scholar
  12. Kowluru, R.A., Engerman, D.L., Case, G.L., and Kern, T.S., 2001, Retinal glutamate in diabetes and effect of antioxidants.Neurochem. Int. 38:385.PubMedCrossRefGoogle Scholar
  13. Li, Q., and Pouro, D.G., 2002, Diabetes-induced dysfunction of the glutamate transporter in retinal Müller cells.Invest. Ophthalmol Vis. Sci. 43:3109.PubMedGoogle Scholar
  14. Lieth, E., Barber, A., Xu, B., Dice, C., Ratz, M., Tanase, D., and Strother, J., 1998, Glial reactivity and impaired glutamate metabolism in short-term experimental diabetic retinopathy.Diabetes 42:815.CrossRefGoogle Scholar
  15. Lieth, E., Lanoue, K.F., Antonetti, D.A., Ratz, M., and The Penn State Retina Research Group, 2000, Diabetes reduces glutamate oxidation and glutamine synthesis in the retina.Exp. Eye Res.70: 723.CrossRefGoogle Scholar
  16. Lu, M., Perez, V.L., Naili, M., Miyamoto, K., Peng, H.-B., Liao, J.K., and Adamis, A.P., 1999, VEGF increases retinal vascular ICAM-1 expression in vivo.Invest. Ophthalmol. Vis. Sci. 40:1808.PubMedGoogle Scholar
  17. Miyamoto, K., Khosrof, S., Bursell, S.-E., Moromizato, Y., Aiello, L.P., Ogura, Y., and Adamis, A.P., 2000, Vascular endothelial growth factor (VEGF)-induced retinal vascular permeability is mediated by intercellular adhesion molecule-1 (ICAM-1).Am. J. Pathol. 156:1733.PubMedCrossRefGoogle Scholar
  18. Miyamoto, K., Khosrof, S., Bursell, S.-E., Rohan, R., Murata, T., Clermont, A.C., Aiello, L.P., Ogura, Y., and Adamis, A.O., 1999, Prevention of leukostasis and vascular leakage in streptozotocin-induced diabetic retinopathy via intercellular adhesion molecule-1 inhibition.Proc. Natl. Acad. Sci. 96:10836.PubMedCrossRefGoogle Scholar
  19. Poitry, S., Poitry-Yamate, C., Ueberfeld, J., MacLeish, P.R., and Tsacopoulos, M., 2000, Mechanisms of glutamate metabolic signaling in retinal glial (Müller) cells. J.Neurosci. 20:1809.PubMedGoogle Scholar
  20. Qaum, T., Xu, Q., Joussen, A.M., Clemens, M.W., Qin, W., Miyamoto, K., Hassessian, H., Wiegand, S.J., Rudge, J., Yancopoulos, G.D., and Adamis, A.P., 2001, VEGF-initiated blood-retinal barrier breakdown in early diabetes.Invest. Ophthalmol. Vis. Sci. 42:2408.PubMedGoogle Scholar
  21. Qu, H., Eloqayli, H., Unsgard, G., and Sonnewald, U., 2001, Glutamate decreases pyruvate carboxylase activity and spares glucose as energy substrate in cultured cerebellar astrocytes.I Neurosci. Res. 66:1127.CrossRefGoogle Scholar
  22. Rungger-Brandle, E., Kolb, H., and Niemeyer, G., 1996, Histochemical demonstration of glycogen in neurons of the cat retina.Invest. Ophthalmol. Vis. Sci. 37: 702.PubMedGoogle Scholar
  23. Rungger-Brändle, E., Dosso, A.A., and Leuenberger, P.M., 2000, Glial reactivity, an early feature of diabetic retinopathy.Invest. Ophthalmol. Vis. Sci. 41:1971.PubMedGoogle Scholar
  24. Rybicka, K.K., 1996, Glycosomes - the organelles of glycogen metabolism.Tissue and Cell 28:253.PubMedCrossRefGoogle Scholar
  25. Takeo-Goto, S., Doi, M., Ma, N., Goto, R., Semba, R., and Uji, Y., 2002, Immunohistochemical localization of amino acids in the diabetic retina of goto-kakizaki rats.Ophthalmic Res. 34:139.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2003

Authors and Affiliations

  • Elisabeth Rungger-Brändle
    • 1
  • André A. Dosso
    • 1
  1. 1.Cell Biology LaboratoryUniversity Eye ClinicGeneva 14Switzerland

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