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Applications of in Vivo EPR Spectroscopy and Imaging to Skin

  • Jürgen Fuchs
  • Norbert Groth
  • Thomas Herrling
Part of the Biological Magnetic Resonance book series (BIMR, volume 18)

Abstract

Electron paramagnetic resonance (EPR) spectroscopy and imaging of free radicals in human skin performed at low-frequency (3 GHz) continuous-wave EPR is a promising technique to study redox metabolism, formation of free radicals and reactive oxygen species, oxygen concentration, barrier function and drug penetration in vivo. The method relies on the application of nitroxides, nitroxide labeled drugs or nitrone spin traps. Pyrrolidine and piperidine type nitroxides have a low potential to cause dermatotoxic effects such as irritant or allergic contact dermatitis and can possibly be used safely for topical in vivo studies. Dermatologic applications of the EPR technique are discussed including in vitro studies, which show the potential for in vivo investigations.

Keywords

Electron Paramagnetic Resonance Human Skin Stratum Corneum Spin Label Free Radical Formation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 2003

Authors and Affiliations

  • Jürgen Fuchs
    • 1
  • Norbert Groth
    • 2
  • Thomas Herrling
    • 3
  1. 1.Department of Dermatology, Medical SchoolJ. W. Goethe UniversityFrankfurtGermany
  2. 2.Galenus GmbHBerlinGermany
  3. 3.FOM InstituteTFH Berlin - University of Applied Science and TechnologyBerlinGermany

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