Sample Analysis and Data Processing
The application of a selected reaction monitoring method in a complete, multiplexed assay is achieved by combining multiple descriptors. Care must be taken to find an optimal number of targets so that proper sampling of the chromatographic experiment is still obtained. The final challenge is then analyzing the data to determine the signals for the respective peptides and calculating an amount of each protein. Internal standards and housekeeping proteins facilitate this process.
KeywordsChromatographic Peak Select Reaction Monitoring Fragmentation Reaction Quantitative Proteomics Mass Spectrometry Experiment
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