Mass Spectrometry

  • Igor A. KaltashovEmail author
  • Cedric E. Bobst
Part of the Biophysics for the Life Sciences book series (BIOPHYS, volume 6)


Mass spectrometry is now an indispensable tool in the armamentarium of molecular biophysics, where it is used for tasks ranging from covalent structure determination to studies of higher order structure, conformational dynamics, and interactions of proteins and other biopolymers. This chapter considers the basics of biological mass spectrometry and highlights recent advances in this field (with particular emphasis on hydrogen exchange, chemical cross-linking, and native electrospray ionization mass spectrometry), evaluates current challenges, and reviews possible future developments.


High Order Structure Charge State Distribution Quadrupole Mass Spectrometry Deuterium Content Mass Spectrometry Measurement 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



Preparation of this manuscript was supported in part by a grant from the National Institutes of Health R01 GM061666. The authors are grateful to Prof. Wendell P. Griffith (University of Toledo), Dr. Rinat R. Abzalimov (University of Massachusetts-Amherst), Dr. Guanbo Wang (Universiteit Utrecht), and Dr. Shunhai Wang (Regeneron, Inc.) for sharing unpublished data sets for this manuscript.


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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  1. 1.Department of ChemistryUniversity of Massachusetts-AmherstAmherstUSA

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