Optical Spectroscopic Methods for the Analysis of Biological Macromolecules

  • Linda O. NarhiEmail author
  • Cynthia H. Li
  • Ranjini Ramachander
  • Juraj Svitel
  • Yijia Jiang
Part of the Biophysics for the Life Sciences book series (BIOPHYS, volume 6)


The interaction of light with macromolecules is used in multiple different ways in the life sciences. These interactions can be exploited to learn about the structure, stability, and function of proteins and nucleic acids. In this chapter we cover analysis of macromolecules, primarily at equilibrium, by UV absorbance, circular dichroism, fluorescence, Fourier transform infrared, and Raman spectroscopies, and light scattering. A brief description of the underlying theory and some examples of applications are provided for each technique.


UV absorbance Fluorescence spectroscopy Fourier transform infrared (FTIR) spectroscopy Raman spectroscopy Circular dichroism Light scattering Spectroscopy 


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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Linda O. Narhi
    • 1
    Email author
  • Cynthia H. Li
    • 1
  • Ranjini Ramachander
    • 1
  • Juraj Svitel
    • 1
  • Yijia Jiang
    • 1
  1. 1.Research and Development, Amgen, Inc.Thousand OaksUSA

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