Preclinical Imaging in BSL-3 and BSL-4 Environments: Imaging Pathophysiology of Highly Pathogenic Infectious Diseases

  • Lauren Keith
  • Svetlana Chefer
  • Laura Bollinger
  • Jeffrey Solomon
  • Srikanth Yellayi
  • Jurgen Seidel
  • David Thomasson
  • Peter Jahrling
Part of the AAPS Advances in the Pharmaceutical Sciences Series book series (AAPS, volume 8)


Structural and functional imaging are emerging as powerful tools for studying highly pathogenic infectious disease processes. Nuclear imaging modalities and sophisticated radiolabeled probes can be used to track physiological or biochemical processes associated with viral infection. Magnetic resonance imaging can provide anatomical images with exquisite soft tissue contrast, while magnetic resonance spectroscopy can measure the relative amounts of certain metabolites in a given tissue. However, conducting medical imaging studies in a high-containment laboratory requires advanced applications and modification not only of image acquisition and analysis processes but also of the imaging equipment. Processes such as ex vivo labeling of cells are hampered by the personal protective equipment required for the safety of laboratory personnel. Modification of medical imaging equipment can prevent contamination of the equipment. Regardless of the challenges involved, medical imaging could provide valuable information to researchers developing therapeutics against highly pathogenic infectious diseases.


West Nile Virus Personal Protective Equipment Imaging Equipment Service Engineer Containment Strategy 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



The authors thank Fabian de Kok-Mercado for his contribution to this work. The content of this chapter does not necessarily reflect the views or policies of the US Department of Health and Human Services or of the institutions and companies affiliated with the authors. This work was supported by the Integrated Research Facility, Division of Clinical Research, National Institute of Allergy and Infectious Diseases (NIAID), and Battelle Memorial Institute’s prime contract with NIAID (HHSN272200200016I).


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Copyright information

© American Association of Pharmaceutical Scientists 2014

Authors and Affiliations

  • Lauren Keith
    • 1
  • Svetlana Chefer
    • 1
  • Laura Bollinger
    • 1
  • Jeffrey Solomon
    • 1
  • Srikanth Yellayi
    • 1
  • Jurgen Seidel
    • 1
  • David Thomasson
    • 1
  • Peter Jahrling
    • 1
    • 2
  1. 1.Integrated Research Facility, National Institutes of Allergy and Infectious Diseases, National Institutes of HealthFrederickUSA
  2. 2.Emerging Viral Pathogens SectionNational Institute of Allergy and Infectious Diseases, National Institutes of HealthBethesdaUSA

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