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Allele Discovery Platform (ADP) in Papaya (Carica papaya L.)

  • P. K. Anish Kumar
  • Anjanabha Bhattacharya
  • O. P. Dutta
  • Manash ChatterjeeEmail author
Chapter
Part of the Plant Genetics and Genomics: Crops and Models book series (PGG, volume 10)

Abstract

Papaya (Carica papaya L.) is a soft-wooded herbaceous dicotyledonous plant that belongs to the family Caricaceae. Papaya ranks first among fruits consumed and also ranks first in nutritional profile. Besides its nutritional and medicinal properties, papaya has a number of characteristics that contribute to its being used as an experimental model for tree crops. Papaya is among the limited number of plant species that are trioecious with three sex forms—female, male, and hermaphrodite—making it an excellent model for basic studies on sex determination. In recent times, papaya has tremendously benefited from genomics and especially transgenic technology. However, resistance to transgenic technology has pushed scientists to look for non-transgenic methods for its improvement. TILLING (targeting induced local lesion in genome)/ADP (allele discovery platform) is an alternative non-transgenic tool for creating variability in crop species. In this chapter, we will present data to demonstrate that TILLING can be used successfully for papaya breeding and basic functional genomics studies.

Keywords

High Resolution Melting Tobacco Etch Virus Hermaphrodite Flower Carica Papaya Cyanogenic Glucoside 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

We would like to thank Mr Uday Singh and Dr N. Anand (Namdhari Seeds) for financial support and encouragement. We also thank Dr A. Bendahmane (URGV lab, INRA) for advice on the TILLING approach and PRSV resistance. The contributions of all the glass house and field staff involved with the growing of the mutant populations are hereby acknowledged.

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Website

  1. Source-sink relationship during papaya fruit growth and development. http://www.ctahr.hawaii.edu/t-star/papaya.htm

Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • P. K. Anish Kumar
    • 1
  • Anjanabha Bhattacharya
    • 1
  • O. P. Dutta
    • 2
  • Manash Chatterjee
    • 1
    Email author
  1. 1.Bench Bio Pvt LtdVapiIndia
  2. 2.Department of Research and DevelopmentNamdhari Seeds Pvt LtdBangaloreIndia

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