Physical Mapping of Papaya Sex Chromosomes

  • Jianping WangEmail author
  • Jong-Kuk Na
  • Ray Ming
Part of the Plant Genetics and Genomics: Crops and Models book series (PGG, volume 10)


Papaya sex chromosomes have recently evolved and suitable for studying the early events of sex chromosome evolution that are not detectable in ancient sex chromosomes like those of humans. The papaya hermaphrodite-specific Y chromosome region (HSY) is pericentromeric, heterochromatic, and suppressed for recombination. Complete sequencing and analysis of the papaya sex chromosomes will advance our knowledge of sex determination mechanism(s) in plants and the evolutionary process of young sex chromosomes. Physical mapping of HSY and its X counterpart is essential for complete sequencing of the papaya sex chromosomes. The maps of bacterial artificial chromosome (BAC) in minimal tiling path for both HSY and the corresponding X region were constructed in papaya through chromosome walking. The HSY map contained 68 anchored overlapped BAC clones and spanned approximately 8.5 Mb, while the physical map for the corresponding X region had 44 BAC clones and extended about 5.4 Mb with a small gap in the middle of the map unfilled. The borders of the MSY/X region were defined by fine mapping with a large F2 population. The HSY exhibited about 89 % expansion of DNA sequence compared to the corresponding X region, indicating expansion of the Y chromosome at an early evolutionary stage. These physical maps of HSY and X corresponding region in papaya provide the foundation for sequencing and analysis of the heterochromatic young sex chromosomes in papaya.


Bacterial Artificial Chromosome Bacterial Artificial Chromosome Clone Bacterial Artificial Chromosome Library Chromosome Walking Minimum Tiling Path 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  1. 1.Department of Agronomy, Genetics Institute, Plant Molecular and Cellular Biology ProgramUniversity of FloridaGainesvilleUSA
  2. 2.Department of Agricultural Bio-ResourcesNational Academy of Agricultural Science, RDASuwonRepublic of Korea
  3. 3.Department of Plant BiologyUniversity of Illinois at Urbana-ChampaignUrbanaUSA

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