Plasmodesmata: New Perspectives on Old Questions

  • Robyn L. Overall
  • Danny Y. T. Liu
  • Deborah A. Barton
Chapter

Abstract

The progress so far and challenges remaining in developing a functional model of the macromolecular architecture of plasmodesmata are discussed. Details of the macromolecular components identified within plasmodesmata are summarised. Electron tomography and correlative microscopy techniques are explored as potential avenues to overcome the challenges in developing an accurate three-dimensional model of the macromolecular architecture of plasmodesmata. In recent years, some areas of plasmodesmatal biology have been left ignored, largely because the technologies required to advance them have been considered too difficult. For example, there have been no electrophysiological studies of plasmodesmata in the last decade and consequently no advances in our understanding of the rapid regulation of the permeability of plasmodesmata. There has also been no advance on the question of heterogeneity of function of the plasmodesmata within a wall and potential avenues to address this question are considered.

Keywords

Correlative microscopy Cytoskeleton Electron tomography Electrophysiology Endoplasmic reticulum Macromolecular architecture Plasmodesmata Sphincter 

Abbreviations

CMV

Cucumber mosaic virus

ER

Endoplasmic reticulum

FA

Functional assay

FM

Fluorescence microscopy

FPF

Fluorescent protein fusion observed using fluorescence microscopy

GFP

Green fluorescent protein

GPI

Glyco phosphatidyl inositol

IFM

Immunofluorescence microscopy

ITEM

Immune-gold transmission electron microscopy

MPTMV

Movement protein of Tobacco mosaic virus

MPTVCV

Movement protein of Turnip vein-clearing mosaic virus

NCAP

Non-cell autonomous protein

PA

Protein analyses such as immune-blotting and proteomics

PD

Plasmodesmata/plasmodesma

RFP

Red fluorescent protein

ROS

Reactive oxygen species

SEL

Size exclusion limit

TEM

Transmission electron microscopy

YFP

Yellow fluorescent protein

Notes

Acknowledgements

RLO thanks the Australian Research Council for financial support and DYTL thanks the Grains Research and Development Corporation for a postgraduate research award.

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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Robyn L. Overall
    • 1
  • Danny Y. T. Liu
    • 2
  • Deborah A. Barton
    • 1
  1. 1.School of Biological SciencesThe University of SydneyCamperdownAustralia
  2. 2.School of Biological SciencesThe University of SydneyCamperdownAustralia

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