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Application of Baculovirus Technology for Studies of G Protein-Coupled Receptor Signaling

Part of the Springer Proceedings in Physics book series (SPPHY,volume 146)

Abstract

G protein-coupled receptors (GPCR), also referred to as 7 transmembrane spanning receptors (7TM), constitute the largest superfamily of receptors in vertebrates and invertebrates. These receptors respond to a wide range of signaling compounds such as bioamines, lipids, peptides, hormones, odorants, and even photons. They mediate the majority of cell-to-cell communication in the human body and targets for nearly one third of the prescription drugs on the market.

Keywords

  • GPCR Signaling
  • Baculovirus Expression Vector System
  • Fluorescent Nucleotide
  • Orthosteric Binding Site
  • Multicapsid Nucleopolyhedrovirus

These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Acknowledgments

We thank Professor Martin J. Lohse’s group at the University of Würzburg for providing us the Epac-camp sensor plasmid. We thank Dr. Kees Jalink’s group at the Netherlands Cancer Institute for providing us the TEpacVV sensor plasmid.

The work was funded by the Estonian Science Foundation (7569, 8011, 8314) by the Estonian Ministry of Education and Science (SF0180032s12) and by the European Union through the European Regional Development Fund (TK114, 30020).

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Correspondence to Sergei Kopanchuk .

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Mazina, O., Tõntson, L., Veiksina, S., Kopanchuk, S., Rinken, A. (2013). Application of Baculovirus Technology for Studies of G Protein-Coupled Receptor Signaling. In: Fesenko, O., Yatsenko, L., Brodin, M. (eds) Nanomaterials Imaging Techniques, Surface Studies, and Applications. Springer Proceedings in Physics, vol 146. Springer, New York, NY. https://doi.org/10.1007/978-1-4614-7675-7_23

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  • DOI: https://doi.org/10.1007/978-1-4614-7675-7_23

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