Imaging Living Cells and Mapping Their Surface Molecules with the Atomic Force Microscope
In 1986 Gerd Binnig and Heinrich Roher shared the Nobel Prize in Physics for inventing the scanning tunneling microscope (STM) and discovering that it can image individual atoms with unprecedented resolution (Binnig et al., 1982). This novel type of microscopy is based on the quantum phenomenon that electrons can tunnel through a narrow insulating gap between two conductors.
KeywordsAgar Polysaccharide Shrinkage Immobilization Nitride
Unable to display preview. Download preview PDF.
- Bezanilla, M., C. J. Bustamante, and H. G. Hansma. Improved visualization of DNA in aqueous buffer with the atomic force microscope. Scanning Microsc. 7 (4): 1145–1148, 1993.Google Scholar
- Cooper, S. Bacterial Growth and Division. San Diego: Academic Press, 1991, p. 359.Google Scholar
- Farkas, V., J. Kovarfk, A. Kosinovâ, and S. Bauer. Autoradiographic study of mannan incorporation into the growing cell walls of Saccharomyces cerevisiae J. Bacteriol. 117: 265–269, 1974.Google Scholar
- Häberle, W., J. K. H. Hörber, F. Ohnesorge, D. P. E. Smith, and G. Binnig. In situ investigation of single living cell infected by viruses. Ultramicroscopy 42–44:1161–1167, 1992.Google Scholar
- Israelachvili, J. N. Intermolecular and Surface Forces. London: Academic Press, 1992.Google Scholar
- Shroff, S. G., D. R. Saner, and R. Lal. Atomic force microscopy of arterial cells: Local viscoelastic mechanical-properties and imaging of cytoskeleton. Biophys. J. 66: 278a, 1994.Google Scholar