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Molecular Hydrogen Consumption in the Human Body During the Inhalation of Hydrogen Gas

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Oxygen Transport to Tissue XXXV

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 789))


Inhaling or ingesting hydrogen (H2) gas improves oxidative stress-induced damage in animal models and humans. We previously reported that H2 was consumed throughout the human body after the ingestion of H2-rich water and that the H2 consumption rate (\( {\text{V}}_{{\text{H}}_{2}}\)) was 1.0 μmol/min/m2 body surface area. To confirm this result, we evaluated \( {\text{V}}_{{\text{H}}_{2}}\)during the inhalation of low levels of H2 gas. After measuring the baseline levels of exhaled H2 during room air breathing via a one-way valve and a mouthpiece, the subject breathed low levels (160 ppm) of H2 gas mixed with purified artificial air. The H2 levels of their inspired and expired breath were measured by gas chromatography using a semiconductor sensor. \( {\text{V}}_{{\text{H}}_{2}}\) was calculated using a ventilation equation derived from the inspired and expired concentrations of O2/CO2/H2, and the expired minute ventilation volume, which was measured with a respiromonitor. As a result, \( {\text{V}}_{{\text{H}}_{2}}\) was found to be approximately 0.7 μmol/min/m2BSA, which was compatible with the findings we obtained using H2-rich water. \( {\text{V}}_{{\text{H}}_{2}}\) varied markedly when pretreatment fasting to reduce colonic fermentation was not employed, i.e., when the subject’s baseline breath hydrogen level was 10 ppm or greater. Our H2 inhalation method might be useful for the noninvasive monitoring of hydroxyl radical production in the human body.

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This study was supported by the Japan Society for the Promotion of Science (Grant-in-Aid 21240057, 21659211, 24659288) and the Intramural Research Fund of the National Cerebral and Cardiovascular Center (22-4-5, 22-1-5). The authors have no conflicts of interest to report. We thank the volunteers who participated in this study.

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Correspondence to Akito Shimouchi .

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Shimouchi, A., Nose, K., Mizukami, T., Che, DC., Shirai, M. (2013). Molecular Hydrogen Consumption in the Human Body During the Inhalation of Hydrogen Gas. In: Van Huffel, S., Naulaers, G., Caicedo, A., Bruley, D.F., Harrison, D.K. (eds) Oxygen Transport to Tissue XXXV. Advances in Experimental Medicine and Biology, vol 789. Springer, New York, NY.

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