A Cell Culture System with Programmable Physical Stimulation for Human Mesenchymal Stem Cell Differentiation and Promotion
It is important to study the osteoblast and chondrocyte differentiations of human mesenchymal stem cells (hMSCs) due to a significant demand for prerequisite regenerative medicine. Many physical stimulators would also promote cell differentiation. This study presents a time-efficient cell differentiation system to induce and evaluate the stem cell differentiation process under given physical stimulations. In this system, all the driving signals of physical stimulations can be controlled independently by a reprogrammable microcomputer for physical stimulation studies. This chapter focuses on the design methodology and characterization of this cell culture system.
KeywordshMSCs Cell culture Programmable Physical stimulation
- 1.National Center for Biotechnology Information. http://www.ncbi.nlm.nih.gov/
- 3.McCullen SD, McQuilling JP, Grossfeld RM, Lubischer JL, Clarke LI, Loboa EG (2010) Application of low-frequency alternating current electric fields via interdigitated electrodes: effects on cellular viability, cytoplasmic calcium, and osteogenic differentiation of human adipose-derived stem cells. Tissue Eng Part C Method 16(6):1377–1386CrossRefGoogle Scholar
- 5.Cohen DM, Chen CS (2008) Mechanical control of stem cell differentiation. StemBook. Harvard Stem Cell Institute, Cambridge, MA. http://www.ncbi.nlm.nih.gov/pubmed/20614621