Noninvasive NMR and NIRS Measurement of Vascular and Intracellular Oxygenation In Vivo

  • Youngran Chung
  • Thomas Jue
Part of the Handbook of Modern Biophysics book series (HBBT, volume 4)


In the canonical biochemical view, respiration requires a coordinate regulation of oxygen transport, carbon unit flux, electron flow, proton pumping, and ADP translocation. Certainly in-vitro experiments have supported the paradigm, cast in terms of either a kinetic or thermodynamic model. How the cell mobilizes its metabolic resources, increases its metabolic rates, and adjusts O2/nutrient flux still pose questions about the currently accepted theory of respiratory control. Although some researchers downplay a significant role for a metabolic regulation of VO2 and point to a tight match between O2 supply throughout contraction, others have contended that metabolism must modulate VO2 demand and blood flow. Resolving these discordant viewpoints requires an accurate assessment of intracellular VO2 and the O2 supply. Because whole body VO2 and arterial venous oxygen difference measurements with a mean end capillary blood flow have limited accuracy in determining the dynamic change in VO2 in localized myocytes, researchers have explored noninvasive in-vivo techniques, such as near-infrared spectroscopy (NIRS) and nuclear magnetic resonance (NMR).


Nuclear Magnetic Resonance NIRS Spectrum Isosbestic Point Heme Pocket NIRS Signal 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



We would like to acknowledge funding support from NIH GM 58688, and the Japan Society for the Promotion of Science, Bilateral Programs (7301001471) for help in writing the chapter and in obtaining some of the data described herein.


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© Springer Science+Business Media New York 2013

Authors and Affiliations

  1. 1.Biochemistry and Molecular MedicineUniversity of California DavisDavisUSA

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