Abstract
β-Mannanase production in batch solid-state fermentation (SSF) of palm kernel cake (PKC) was evaluated with flasks and a laterally aerated moving bed (LAMB) bioreactor using Aspergillus flavus UMS01. Optimum condition for flask SSF was 110 % moisture content, initial pH 6, 30 °C and particle size 855 μm, yielding 383 U g−1 dry PKC after 120 h. Under the same condition and particle size <5 mm, SSF in LAMB produced 276 U g−1 dry PKC at an optimal gas flow of 4.4 m s−1 in just 96 h. Optimal β-mannanase production was consistent with highest fungal growth and mannose production; to support increasing fungal growth, secretion of β-mannanase increased to degrade mannans in PKC, producing mannose for microbial consumption. A. flavus UMS01 showed promising attributes as a β-mannanase producer via SSF of PKC in flasks and LAMB bioreactor.
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Acknowledgments
Financial support came from Universiti Malaysia Sabah Fundamental Research Grant B-0101-13/PRU037 and PKC was provided by IOI Edible Oils Sdn. Bhd.. All experimental works were performed in Universiti Malaysia Sabah.
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Wong, Y.P., Saw, H.Y., Janaun, J., Krishnaiah, K., Prabhakar, A. (2013). β-Mannanase Production by Aspergillus flavus in Solid-State Fermentation of Palm Kernel Cake. In: Pogaku, R., Bono, A., Chu, C. (eds) Developments in Sustainable Chemical and Bioprocess Technology. Springer, Boston, MA. https://doi.org/10.1007/978-1-4614-6208-8_48
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DOI: https://doi.org/10.1007/978-1-4614-6208-8_48
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