Cynodon dactylon (L.) Pers. is a type of perennial grass that possesses great medicinal values. In this study, the antimicrobial activity of the plant crude extract from seven different solvents (acetone, chloroform, diethyl ether, ethanol, ethyl acetate, methanol, and n-pentane) was investigated against some pathogens (Bacillus cereus, Bacillus subtilis, Escherichia coli, Klebsiella spp., Pseudomonas aeruginosa, Staphylococcus aureus, Streptococcus pyogenes, and Streptococcus pneumonia) using disc diffusion method and thin-layer chromatographic (TLC) bioassay for plant-SPE extracts against Aspergillus niger. Crude extraction showed that ethanolic extraction produced highest yield (7.065 %) followed by methanolic (5.420 %) and chloroform (3.550 %) extraction. The lowest yield was obtained from n-pentane extraction (0.500 %). The antimicrobial study revealed broad spectrum of antimicrobial activity from ethanol (7.0–10.0 ± 0.0–1.0 mm) and ethyl acetate (7.0–12.0 ± 0.0–1.0 mm) extracts against all of the bacterial pathogens. Both methanol and acetone extracts showed activity to B. cereus (8.0 ± 0.0 mm) and B. subtilis (7.0 ± 0.0 mm), while chloroform extract showed activity to B. subtilis (7.0 ± 0.0 mm) and S.pyogenes (8.3 ± 0.6 mm), respectively. Diethyl ether extraction showed activity only to S. pyogenes (7.3 ± 0.6 mm), while no activity was observed from n-pentane extraction. Great antimicrobial activity were observed for both ethyl acetate and ethanol SPE-based extracts (SBE) with size of inhibition ranging from 8.0 ± 0.0 mm to 15.7 ± 0.6 mm for ethyl acetate SBE and 8.0 ± 0.0 mm to 13.0 ± 0.0 mm for ethanol SBE. No significant antimicrobial activity was observed from thin-layer chromatographic bioassay against A. niger.
- Cynodon dactylon
- Crude extract
- SPE-based extract
- TLC bioassay
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Adalet, A., Cenk, A., Azap, A., Onder, E., & Ismail, B. (2011). The impact of a nationwide antibiotic restriction program on antibiotic usage and resistance against nosocomial pathogens in Turkey. International Journal of Medical Science, 4, 339–344.
Albert-Baskar, A., & Ignacimuthu, A. (2010). Chemopreventive effect of Cynodon dactylon (L.) Pers. extract against DMH-induced colon carcinogenesis in experimental animals. Experimental and Toxicology Pathology, 62, 423–431.
Chaudhari, Y., Mody, H. R., & Acharya, V. B. (2011). Antibacterial activity of Cynodon dactylon on different bacterial pathogens isolated from clinical samples. International Journal of Pharmaceutical Studies and Research, 1, 16–20.
Chong, K. P., Rossall, S., & Atong, M. (2011). HPLC Fingerprints and in vitro antimicrobial activity of syringic acid, caffeic acid and 4-hydroxybenzoic acid against Ganoderma boninense. Journal of Applied Sciences, 13, 2284–2291.
Francisco, I. A., & Pinotti, M. H. P. (2000). Cyanogenic glycosides in plants. Brazilian Archives of Biology & Technology, 43, 487–492.
Joy, P. P., Thomas, J., Mathew, J., & Skaria, B. P. (2001). Medicinal Plants. In: T. K. Bose, J. Kabir, P. Das, & P. P. Joy (eds.) Tropical Horticulture, Vol. 2. Calcutta: Naya Prokash.
Kafaru, E. (1994). Immense help from Nature’s Workshop. Lagos: Elikat Health Services.
Katewa, S. S., Guria, B. D., & Jain, A. (2001). Ethnomedicinal and obnoxious grasses of Rajasthan, India. Journal of Ethnopharmacology, 76, 293–297.
Lalitha, M. K. (2011). Methods of antimicrobial susceptibility testing in Manual on antimicrobial susceptibility testing. Vellore: Department of Microbiology, Christian Medical College.
Madigan, M. T., Martinko, J. M., Dunlap, P. V., & Clark, D. P. (2009). The principles of bacterial cell wall In Brock Biology of Microorganisms (12th ed.). San Francisco: Pearson Benjamin Cummings.
Mandal, V., Mohan, Y., & Hemalatha, S. (2007). Microwave assisted extraction—an innovative and promising extraction tool for medicinal plant research. PHCOG REV: Review article. Pharmacognosy Reviews, 1, 7–17.
Mangathayaru, K., Umadevi, M., & Reddy, C. U. (2009). Evaluation of the immunomodulatory and DNA protective activities of the shoots of Cynodon dactylon. Journal of Ethnopharmacology, 123, 181–184.
Najafi, M., Nazemiyeh, H., Ghavimi, H., Gharakhani, A., & Garjani, A. (2008). Effects of the hydroalcoholic extract of Cynodon dactylon (L.) pers. on ischemia/reperfusion-induced arrhythmias. DARU: Journal of Pharmaceuticals Science, 16, 233–238.
Nobmann, P., Bourke, P., Dunne, J., & Henehan, G. (2009). In vitro antimicrobial activity and mechanism of action of novel carbohydrate fatty acid derivatives against Staphylococcus aureus and MRSA. Journal of Applied Microbiology, 108, 2152–2161.
Pongsak, R., & Parichat, P. (1994). Contents and antibacterial activity of flavonoids extracted from leaves of Psidium guajava. Journal of Medicinal Plants Research, 5, 393–396.
Robbins, C. T., Hanley, T. A., Hagerman, A. E., Hjeljord, O., Baker, D. L., & Schwartz, C. C. (1987). Role of tannins in defending plants against ruminants: Reduction in protein availability. Ecology, 68, 98–107.
Sadki, C., Hacht, B., Souliman, A., & Atmani, F. (2010). Acutes diuretic activity of aqueous Erica multiflora flowers and Cynodon dactylon rhizomes extracts in rats. Journal of Ethnopharmacology, 128, 352–356.
Singh, R., & Gupta, A. (2008). Antimicrobial and antitumor activity of the fractionated extracts of Kuli musli (Curculg oorchioides). International Journal of Green Pharmacy, 1, 34–36.
Singh, S. K., Kesari, A. N., Gupta, R. K., Jaiswal, D., & Watal, G. (2007). Assessment of antidiabetic potential of Cynodon dactylon extract in streptozotocin diabetic rats. Journal of Ethnopharmacology, 114, 174–179.
Syahriel, A., Gobilik, G., & Chong, K. P. (2012). Preliminary phytochemical study and antimicrobial activity from various extract of Cynodon dactylon (L.) Pers. (Bermuda) against selected pathogens. International Journal of Pharmacy Pharmaceutical Science, 4, 227–230.
Watson, L., & Dallwitz, M. J. (1992). The grass genera of the world. Wallingford: CAB International.
Wu, Y. Q., Taliaferro, C. M., Martin, D. L., Anderson, J. A., & Anderson, M. P. (2007). Genetic variability and relationships for adaptive, morphological, and biomass traits in Chinese Bermuda grass accessions. Crop Science, 47, 1985–1994.
Yadav, Y. C., Jain, A., & Deb, L. (2010). Neuropharmacological screening techniques for pharmaceuticals—a review. International Journal of Pharmacy Pharmaceutical Science, 2, 10–11.
Zwenger, S., & Basu, C. (2008). Plant terpenoids: applications and future potentials. Biotechnology and Molecular Biology Review, 3, 1–7.
The authors acknowledge their profound gratitude to Ministry of Higher Education (MOHE), Malaysia for funding the research (Grant code FRG0288) and the School of Science and Technology, Universiti Malaysia Sabah for providing the facilities for research work. We are indebted to Dr. Katrina and the team, Department of Pathology, Queen Elizabeth Hospital for their support in supplying the biological specimens.
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Abdullah, S., Gobilik, J., Chong, K.P. (2013). In Vitro Antimicrobial Activity of Cynodon dactylon (L.) Pers. (bermuda) Against Selected Pathogens. In: Pogaku, R., Bono, A., Chu, C. (eds) Developments in Sustainable Chemical and Bioprocess Technology. Springer, Boston, MA. https://doi.org/10.1007/978-1-4614-6208-8_29
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