Skip to main content
SpringerLink
Log in
Book cover

Taurine 8 pp 247–257Cite as

Effect of Taurine Chloramine on Differentiation of Human Preadipocytes into Adipocytes

  • Conference paper
  • First Online:

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 775))

Abstract

We investigated whether taurine chloramine (TauCl), which is ­endogenously produced by immune cells such as macrophages that infiltrate adipose tissue, affects the differentiation of preadipocytes into adipocytes or modulates the expression of adipokines in adipocytes. To study the physiological effects of TauCl on human adipocyte differentiation and adipokine expression, preadipocytes were cultured under differentiation conditions for 14 days in the presence or the absence of TauCl. Differentiated adipocytes were also treated with TauCl in the presence or the absence of IL-1β (1 ng/ml) for 7 days. The culture supernatants were analyzed for adipokines such as adiponectin, leptin, IL-6, and IL-8. At concentrations of 400–600 μM, TauCl significantly inhibited the differentiation of human preadipocytes into adipocytes in a dose-dependent manner. It did not induce the dedifferentiation of adipocytes or inhibit fat accumulation in adipocytes. Expression of major transcription factors of adipogenesis and adipocyte marker genes was decreased after treatment with TauCl, in agreement with its inhibition of ­differentiation. These results suggest that TauCl may inhibit the differentiation of ­preadipocytes into adipocytes. Thus, TauCl or more stable derivatives of TauCl could potentially be a safe drug therapy for obesity-related diseases.

This is a preview of subscription content, log in via an institution.

Chapter
USD   29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   169.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD   219.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD   219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

Abbreviations

MSCs:

Mesenchymal stem cells

C/EBP:

CCAAT/enhancer-binding protein

PPARg:

Peroxisome proliferator-activated receptor γ

FABP:

Fatty acid-binding protein

LPL:

Lipoprotein lipase

GLUT4:

Glucose transporter 4

TauCl:

Taurine chloramine

HOCl:

Hypochlorous acid

References

  • Apovian CM, Bigornia S, Mott M, Meyers MR, Ulloor J, Gagua M, McDonnell M, Hess D, Joseph L, Gokce N (2008) Adipose macrophage infiltration is associated with insulin resistance and vascular endothelial dysfunction in obese subjects. Arterioscler Thromb Vasc Biol 28: 1654–1659

    Article  PubMed  CAS  Google Scholar 

  • Ariel A, Serhan CN (2007) Resolvins and protectins in the termination program of acute inflammation. Trends Immunol 28:176–183

    Article  PubMed  CAS  Google Scholar 

  • Bjorntorp P, Berchtold P, Tibblin G (1971) Insulin secretion in relation to adipose tissue in men. Diabetes 20:65–70

    PubMed  CAS  Google Scholar 

  • Cho EJ, Rahman MA, Kim SW, Baek YM, Hwang HJ, Oh JY, Hwang HS, Lee SH, Yun JW (2008) Chitosan oligosaccharides inhibit adipogenesis in 3T3-L1 adipocytes. J Microbiol Biotechnol 18:80–87

    PubMed  CAS  Google Scholar 

  • Choi HM, Lee YA, Lee SH, Hong SJ, Hahm DH, Choi SY, Yang HI, Yoo MC, Kim KS (2009) Adiponectin may contribute to synovitis and joint destruction in rheumatoid arthritis by stimulating vascular endothelial growth factor, matrix metalloproteinase-1, and matrix metalloproteinase-13 expression in fibroblast-like synoviocytes more than proinflammatory mediators. Arthritis Res Ther 11:R161

    Article  PubMed  Google Scholar 

  • Compher C, Badellino KO (2008) Obesity and inflammation: lessons from bariatric surgery. JPEN J Parenter Enteral Nutr 32:645–647

    Article  PubMed  Google Scholar 

  • Coppack SW (2001) Pro-inflammatory cytokines and adipose tissue. Proc Nutr Soc 60:349–356

    Article  PubMed  CAS  Google Scholar 

  • Gades MD, Stern JS (2005) Chitosan supplementation and fat absorption in men and women. J Am Diet Assoc 105:72–77

    Article  PubMed  CAS  Google Scholar 

  • Gesta S, Tseng YH, Kahn CR (2007) Developmental origin of fat: tracking obesity to its source. Cell 131:242–256

    Article  PubMed  CAS  Google Scholar 

  • Gregoire FM, Smas CM, Sul HS (1998) Understanding adipocyte differentiation. Physiol Rev 78:783–809

    PubMed  CAS  Google Scholar 

  • Halberg N, Wernstedt-Asterholm I, Scherer PE (2008) The adipocyte as an endocrine cell. Endocrinol Metab Clin North Am 37:753–768, x-xi

    Article  PubMed  CAS  Google Scholar 

  • Ho SC, Tai ES, Eng PH, Tan CE, Fok AC (2001) In the absence of dietary surveillance, chitosan does not reduce plasma lipids or obesity in hypercholesterolaemic obese Asian subjects. Singapore Med J 42:6–10

    PubMed  CAS  Google Scholar 

  • Ito T, Schaffer SW, Azuma J (2011) The potential usefulness of taurine on diabetes mellitus and its complications. Amino Acids 42(5):1529–1539

    Article  PubMed  Google Scholar 

  • Itoh M, Suganami T, Hachiya R, Ogawa Y (2011) Adipose tissue remodeling as homeostatic inflammation. Int J Inflam 2011:720926

    PubMed  Google Scholar 

  • Jessen BA, Stevens GJ (2002) Expression profiling during adipocyte differentiation of 3T3-L1 fibroblasts. Gene 299:95–100

    Article  PubMed  CAS  Google Scholar 

  • Kim KS, Park EK, Ju SM, Jung HS, Bang JS, Kim C, Lee YA, Hong SJ, Lee SH, Yang HI, Yoo MC (2007) Taurine chloramine differentially inhibits matrix metalloproteinase 1 and 13 synthesis in interleukin-1beta stimulated fibroblast-like synoviocytes. Arthritis Res Ther 9:R80

    Article  PubMed  Google Scholar 

  • Kirkland JL, Hollenberg CH, Gillon WS (1990) Age, anatomic site, and the replication and differentiation of adipocyte precursors. Am J Physiol 258:C206–C210

    PubMed  CAS  Google Scholar 

  • Klover PJ, Clementi AH, Mooney RA (2005) Interleukin-6 depletion selectively improves hepatic insulin action in obesity. Endocrinology 146:3417–3427

    Article  PubMed  CAS  Google Scholar 

  • MacDougald OA, Mandrup S (2002) Adipogenesis: forces that tip the scales. Trends Endocrinol Metab 13:5–11

    Article  PubMed  CAS  Google Scholar 

  • Ramirez-Zacarias JL, Castro-Munozledo F, Kuri-Harcuch W (1992) Quantitation of adipose conversion and triglycerides by staining intracytoplasmic lipids with Oil red O. Histochemistry 97:493–497

    Article  PubMed  CAS  Google Scholar 

  • Ronti T, Lupattelli G, Mannarino E (2006) The endocrine function of adipose tissue: an update. Clin Endocrinol (Oxf) 64:355–365

    CAS  Google Scholar 

  • Rosen ED, Hsu CH, Wang X, Sakai S, Freeman MW, Gonzalez FJ, Spiegelman BM (2002) C/EBPalpha induces adipogenesis through PPARgamma: a unified pathway. Genes Dev 16:22–26

    Article  PubMed  CAS  Google Scholar 

  • Rosen ED, MacDougald OA (2006) Adipocyte differentiation from the inside out. Nat Rev Mol Cell Biol 7:885–896

    Article  PubMed  CAS  Google Scholar 

  • Rotter V, Nagaev I, Smith U (2003) Interleukin-6 (IL-6) induces insulin resistance in 3T3-L1 adipocytes and is, like IL-8 and tumor necrosis factor-alpha, overexpressed in human fat cells from insulin-resistant subjects. J Biol Chem 278:45777–45784

    Article  PubMed  CAS  Google Scholar 

  • Roufosse CA, Direkze NC, Otto WR, Wright NA (2004) Circulating mesenchymal stem cells. Int J Biochem Cell Biol 36:585–597

    Article  PubMed  CAS  Google Scholar 

  • Schuller-Levis GB, Park E (2004) Taurine and its chloramine: modulators of immunity. Neurochem Res 29:117–126

    Article  PubMed  CAS  Google Scholar 

  • Shehzad A, Ha T, Subhan F, Lee YS (2011) New mechanisms and the anti-inflammatory role of curcumin in obesity and obesity-related metabolic diseases. Eur J Nutr 50:151–161

    Article  PubMed  CAS  Google Scholar 

  • Sowers MR, Karvonen-Gutierrez CA (2010) The evolving role of obesity in knee osteoarthritis. Curr Opin Rheumatol 22:533–537

    Article  PubMed  Google Scholar 

  • Spalding KL, Arner E, Westermark PO, Bernard S, Buchholz BA, Bergmann O, Blomqvist L, Hoffstedt J, Naslund E, Britton T, Concha H, Hassan M, Ryden M, Frisen J, Arner P (2008) Dynamics of fat cell turnover in humans. Nature 453:783–787

    Article  PubMed  CAS  Google Scholar 

  • Szymanski K, Winiarska K (2008) [Taurine and its potential therapeutic application]. Postepy Hig Med Dosw (Online) 62:75–86

    Google Scholar 

  • Thomas EL, Grisham MB, Melton DF, Jefferson MM (1985) Evidence for a role of taurine in the in vitro oxidative toxicity of neutrophils toward erythrocytes. J Biol Chem 260:3321–3329

    PubMed  CAS  Google Scholar 

  • Tsuboyama-Kasaoka N, Shozawa C, Sano K, Kamei Y, Kasaoka S, Hosokawa Y, Ezaki O (2006) Taurine (2-aminoethanesulfonic acid) deficiency creates a vicious circle promoting obesity. Endocrinology 147:3276–3284

    Article  PubMed  CAS  Google Scholar 

  • Weyer C, Foley JE, Bogardus C, Tataranni PA, Pratley RE (2000) Enlarged subcutaneous abdominal adipocyte size, but not obesity itself, predicts type II diabetes independent of insulin resistance. Diabetologia 43:1498–1506

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (2010-0024089 and 2011-0009061).

Author information

Authors and Affiliations

  1. East-West Bone & Joint Disease Research Institute, Kyung Hee University Hospital at Gangdong, 149 Sangil-dong, Gangdong-gu, Seoul, 134-727, Korea

    Kyoung Soo Kim, Hyun-Mi Choi, Hye-In Ji & Myung Chul Yoo

  2. Laboratory for Leukocyte Signaling Research, Department of Pharmacology and BK21 Program, Inha University School of Medicine, Incheon, Korea

    Chaekyun Kim

  3. Department of Pathology, Inje University Sanggye Paik Hospital, Seoul, Korea

    Jung Yeon Kim

  4. Division of Rheumatology, Kyung Hee University Medical School, Seoul, Korea

    Ran Song, So-Mi Kim, Yeon-Ah Lee, Sang-Hoon Lee, Hyung-In Yang & Seung-Jae Hong

Authors
  1. Kyoung Soo Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Hyun-Mi Choi
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Hye-In Ji
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Chaekyun Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Jung Yeon Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Ran Song
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. So-Mi Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Yeon-Ah Lee
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Sang-Hoon Lee
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. Hyung-In Yang
    View author publications

    You can also search for this author in PubMed Google Scholar

  11. Myung Chul Yoo
    View author publications

    You can also search for this author in PubMed Google Scholar

  12. Seung-Jae Hong
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Kyoung Soo Kim or Seung-Jae Hong .

Editor information

Editors and Affiliations

  1. City University of New York, Center for Developmental Neuroscience, College of Staten Island, 2800 Victory Blvd, Staten Island, 10314, New York, USA

    Abdeslem El Idrissi

  2. City University of New York, Department of Biology, College of Staten Island, Victory Blvd 2800, Staten Island, 10314, New York, USA

    William J. L'Amoreaux

Rights and permissions

Reprints and permissions

Copyright information

© 2013 Springer Science+Business Media New York

About this paper

Cite this paper

Kim, K.S. et al. (2013). Effect of Taurine Chloramine on Differentiation of Human Preadipocytes into Adipocytes. In: El Idrissi, A., L'Amoreaux, W. (eds) Taurine 8. Advances in Experimental Medicine and Biology, vol 775. Springer, New York, NY. https://doi.org/10.1007/978-1-4614-6130-2_21

Download citation

Publish with us

Policies and ethics

Search

Navigation