Taurine 8 pp 231-240 | Cite as

Protective Effect of Taurine on Triorthocresyl Phosphate (TOCP)-Induced Cytotoxicity in C6 Glioma Cells

Conference paper
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 776)

Abstract

Triorthocresyl phosphate (TOCP) an organophosphorus ester can cause neurotoxicity via oxidative stress pathway. Taurine is an antioxidant. The objective of this study was to investigate the protective effect of taurine on TOCP-induced cytotoxicity in C6 glioma cell. The C6 glioma cells were pretreated with 0, 1, 3, and 9 mM of taurine for 30 min prior to 1 mM TOCP treatment. After 48 h, cell survival was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and lactate dehydrogenase (LDH) release. The content of glutathione (GSH) and the activity of glutathione peroxidase (GPx) were also analyzed by kits. Our results showed that survival of the glioma cells decreased in the group treated with TOCP alone and increased significantly in the groups pretreated with taurine in a concentration-dependent manner. TOCP induced decrease in the activity of GPx and the content of GSH. However, taurine prevented these decreases. Our results suggested that taurine has protective effect on TOCP-induced toxicity to glioma cells via elevating antioxidant capacity.

Keywords

Toxicity Lactate DMSO Glutathione Shrinkage 

Abbreviations

TOCP

Triorthocresyl phosphate

LDH

Lactate dehydrogenase

GPx

Glutathione peroxidase

GSH

Glutathione

Notes

Acknowledgements

This work was supported by National Nature Science Foundation of China [No. 30771819].

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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  1. 1.Department of Occupational and Environmental HealthDalian Medical UniversityDalianChina

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