Abstract
Understanding the molecular basis of plant responses to the major abiotic stresses such as drought and salinity is very important for the biotechnological application of stress adaptation for crop improvement. In this context, thousands of stress-responsive genes have been identified and a few of them have been functionally characterized. Some of them have been proposed as suitable targets for genetic engineering in order to impart stress tolerance in plants. Amidst numerous genes analyzed, transcription factors are considered to be very good targets for studying the molecular mechanisms of abiotic stress response as they singularly or in conjunction regulate the expression of many downstream target genes. Among the various transcription factor encoding genes, homeobox genes, which are well known to be involved in diverse aspects of development, have also recently been implicated in abiotic stress responses. Through various overexpression and mutant studies, the versatility of homeobox genes in plants has been revealed. There are evidences where these genes have been found to confer stress tolerance in plants. This review highlights the importance of homeobox genes in abiotic stress responses and their potential for engineering stress tolerance for crop improvement.
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References
Agalou A, Purwantomo S, Overnas E et al (2008) A genome-wide survey of HD-Zip genes in rice and analysis of drought-responsive family members. Plant Mol Biol 66:87–103
Aoyama T, Dong CH, Yan WU et al (1995) Ectopic expression of the Arabidopsis transcriptional activator ATHB-1 alters leaf cell fate in tobacco. Plant Cell 7:1773–1785
Arce AL, Raineri J, Capella M, Cabello JV, Chan RL (2011) Uncharacterized conserved motifs outside the HD-Zip domain in HD-Zip subfamily I transcription factors; a potential source of functional diversity. BMC Plant Biol 11:42
Ariel FD, Manavella PA, Dezar CA, Chan RL (2007) The true story of the HD-Zip family. Trends Plant Sci 12:419–426
Bartels D, Sunkar R (2005) Drought and salt tolerance in plants. Crit Rev Plant Sci 24:23–58
Bharathan G, Janssen BJ, Kellogg EA, Sinha N (1997) Did homeodomain proteins duplicate before the origin of angiosperms, fungi, and metazoa? Proc Natl Acad Sci USA 94:13749–13753
Byrne ME, Groover AT, Fontana JR, Martienssen RA (2003) Phyllotactic pattern and stem cell fate are determined by the Arabidopsis homeobox gene BELLRINGER. Development 130:3941–3950
Chan RL, Gago GM, Palena CM, Gonzalez DH (1998) Homeoboxes in plant development. Biochim Biophys Acta 1442:1–19
Chen H, Rosin FM, Prat S, Hannapel DJ (2003) Interacting transcription factors from the three-amino acid loop extension superclass regulate tuber formation. Plant Physiol 132:1391–1404
Chinnusamy V, Schumaker K, Zhu JK (2004) Molecular genetic perspectives on cross-talk and specificity in abiotic stress signaling in plants. J Exp Bot 55:225–236
Choi H, Hong J, Ha J, Kang J, Kim SY (2000) ABFs, a family of ABA-responsive element binding factors. J Biol Chem 275:1723–1730
Cutler SR, Rodriguez PL, Finkelstein RR, Abrams SR (2010) Abscisic acid: Emergence of a core signaling network. Annu Rev Plant Biol 61:651–679
Deng X, Phillips J, Meijer AH, Salamini F, Bartels D (2002) Characterization of five novel Âdehydration responsive homeodomain leucine zipper genes from the resurrection plant Craterostigma plantagineum. Plant Mol Biol 49:601–610
Deng X, Phillips J, Bräutigam A et al (2006) A homeodomain leucine zipper gene from Craterostigma plantagineum regulates abscisic acid responsive gene expression and Âphysiological responses. Plant Mol Biol 61:469–489
Deyhle F, Sarkar AK, Tucker EJ, Laux T (2007) WUSCHEL regulates cell differentiation during anther development. Dev Biol 302:154–159
Dezar CA, Gago GM, Gonzalez DH, Chan RL (2005) Hahb-4, a sunflower homeobox-leucine zipper gene, is a developmental regulator and confers drought tolerance to Arabidopsis Âthaliana plants. Transgenic Res 14:429–440
Dubouzet JG, Sakuma Y, Ito Y et al (2003) OsDREB genes in rice, Oryza sativa L, encode Âtranscription activators that function in drought, high-salt and cold-responsive gene expression. Plant J 33:751–763
Frank W, Phillips J, Salamini F, Bartels D (1998) Two dehydration-inducible transcripts from the resurrection plant Craterostigma plantagineum encode interacting homeodomain leucine Âzipper proteins. Plant J 15:413–421
Fujii H, Verslues PE, Zhu JK (2007) Identification of two protein kinases required for abscisic acid regulation of seed germination, root growth and gene expression in Arabidopsis. Plant Cell 19:485–494
Fujita M, Fujita Y, Maruyama K et al (2004) A dehydration induced NAC protein, RD26, is involved in a novel ABA-dependent stress-signaling pathway. Plant J 39:863–876
Fujita Y, Fujita M, Satoh R et al (2005) AREB1 is a transcription activator of novel ÂABRE-dependent ABA-signaling that enhances drought stress tolerance in Arabidopsis. Plant Cell 17:3470–3488
Fujita M, Fujita Y, Noutoshi Y et al (2006) Crosstalk between abiotic and biotic stress responses: a current view from the points of convergence in the stress signaling networks. Curr Opin Plant Biol 9:436–442
Fujita Y, Fujita M, Shinozaki K, Yamaguchi-Shinozaki K (2011) ABA-mediated transcriptional regulation in response to osmotic stress in plants. J Plant Res 124:509–525. http://dx.doi.org/10.1007/s10265-011-0412-3
Furihata T, Maruyama K, Fujita Y et al (2006) ABA-dependent multisite phosphorylation regulates the activity of a transcription activator AREB1. Proc Natl Acad Sci USA 103:1988–1993
Gago GM, Almoguera C, Jordano J, Gonzalez DH, Chan RL (2002) Hahb-4, a homeobox-leucine zipper gene potentially involved in abscisic acid-dependent responses to water stress in sunflower. Plant Cell Environ 25:633–640
Grant MR, Jones JDG (2009) Hormone (dis)harmony molds plant health and disease. Science 324:750–752
Hadiarto T, Tran LS (2011) Progress studies of drought-responsive genes in rice. Plant Cell Rep 30:297–310
Hake S, Smith HM, Holtan H, Magnani E, Mele G, Ramirez J (2004) The role of knox genes in plant development. Annu Rev Cell Dev Biol 20:125–151
Hay A, Tsiantis M (2010) KNOX genes: Versatile regulators of plant development and diversity. Development 137:3153–3165
Hay A, Craft J, Tsiantis M (2004) Plant hormones and homeoboxes: bridging the gap? Bioessays 26:395–404
Henriksson E, Olsson A, Johannesson H, Hanson J, Engstrom P, Soderman E (2005) Homeodomain leucine zipper class I genes in Arabidopsis expression patterns and phylogenetic relationships. Plant Physiol 139:509–518
Himmelbach A, Hoffmann T, Leube M, Hohener B, Grill E (2002) Homeodomain protein ATHB6 is a target of the protein phosphatase ABI1 and regulates hormone responses in Arabidopsis. EMBO J 21:3029–3038
Hirayama T, Shinozaki K (2007) Perception and transduction of abscisic acid signals: keys to the function of the versatile plant hormone ABA. Trends Plant Sci 12:343–351
Hirayama T, Shinozaki K (2010) Research on plant abiotic stress responses in the post-genome era: past, present and future. Plant J 61:1041–1052
Hjellstrom M, Olsson A, Engstrom P, Soderman E (2003) Constitutive expression of the water deficit-inducible homeobox gene ATHB7 in transgenic Arabidopsis causes a suppression of stem elongation growth. Plant Cell Environ 26:1127–1136
Jain M, Khurana JP (2008) Small RNA regulation of rice homeobox genes. Plant Signal Behav 3:1024–1025
Jain M, Khurana JP (2009) Transcript profiling reveals diverse roles of auxin-responsive genes during reproductive development and abiotic stress in rice. FEBS J 276:3148–3162
Jain M, Tyagi AK, Khurana JP (2008) Genome-wide identification, classification, evolutionary expansion and expression analyses of homeobox genes in rice. FEBS J 275:2845–2861
Johannesson H, Wang Y, Hanson J, Engstrom P (2003) The Arabidopsis thaliana homeobox gene ATHB5 is a potential regulator of abscisic acid responsiveness in developing seedlings. Plant Mol Biol 51:719–729
Kagaya Y, Hobo T, Murata M, Ban A, Hattori T (2002) Abscisic acid induced transcription is mediated by phosphorylation of an abscisic acid response element binding factor, TRAB1. Plant Cell 14:3177–3189
Kasuga M, Liu Q, Miura S, Yamaguchi-Shinozaki K, Shinozaki K (1999) Improving plant drought, salt and freezing tolerance by gene transfer of a single stress-inducible transcription factor. Nat Biotechnol 17:287–291
Kobayashi Y, Murata M, Minami H et al (2005) Abscisic acid-activated SNRK2 protein kinases function in the gene-regulation pathway of ABA signal transduction by phosphorylating ABA response element-binding factors. Plant J 44:939–949
Lee YH, Chun JY (1998) A new homeodomain-leucine zipper gene from Arabidopsis thaliana induced by water stress and abscisic acid treatment. Plant Mol Biol 37:377–384
Luo H, Song F, Zheng Z (2005) Overexpression in transgenic tobacco reveals different roles for the rice homeodomain gene OsBIHD1 in biotic and abiotic stress responses. J Exp Bot 56:2673–2682
Mahajan S, Tuteja N (2005) Cold, salinity and drought stresses: An overview. Arch Biochem Biophys 444:139–158
Manavella PA, Arce AL, Dezar CA (2006) Cross-talk between ethylene and drought signaling pathways is mediated by the sunflower Hahb-4 transcription factor. Plant J 48:125–137
McConnell JR, Emery J, Eshed Y, Bao N, Bowman J, Barton MK (2001) Role of PHABULOSA and PHAVOLUTA in determining radial patterning in shoots. Nature 411:709–713
Mukherjee K, Brocchieri L, Burglin TR (2009) A comprehensive classification and evolutionary analysis of plant homeobox genes. Mol Biol Evol 26:2775–2794
Nakamura M, Katsumata H, Abe M et al (2006) Characterization of the class IV homeodomain-leucine zipper gene family in Arabidopsis. Plant Physiol 141:1363–1375
Nakashima K, Tran LS, Van Nguyen D et al (2007) Functional analysis of a NAC-type transcription factor OsNAC6 involved in abiotic and biotic stress-responsive gene expression in rice. Plant J 51:617–630
Nakashima K, Ito Y, Yamaguchi-Shinozaki K (2009) Transcriptional regulatory networks in response to abiotic stresses in Arabidopsis and grasses. Plant Physiol 149:88–95
Ni Y, Wang X, Li D, Wu Y, Xu W, Li X (2008) Novel cotton homeobox gene and its expression profiling in root development and in response to stresses and phytohormones. Acta Biochim Biophy Sin 40:78–84
Olsson A, Engstrom P, Soderman E (2004) The homeobox genes ATHB12 and ATHB7 encode potential regulators of growth in response to water deficit in Arabidopsis. Plant Mol Biol 55:663–677
Pieterse CMJ, Leon-Reyes A, Van der Ent S, Van Wees SCM (2009) Networking by small-molecule hormones in plant immunity. Nat Chem Biol 5:308–316
Prigge MJ, Otsuga D, Alonso JM, Ecker JR, Drews GN, Clark SE (2005) Class III homeodomain-leucine zipper gene family members have overlapping, antagonistic and distinct roles in Arabidopsis development. Plant Cell 17:61–76
Qin F, Sakuma Y, Li J et al (2004) Cloning and functional analysis of a novel DREB1/CBF transcription factor involved in cold-responsive gene expression in Zea mays L. Plant Cell Physiol 45:1042–1052
Raghavendra AS, Gonugunta VK, Christmann A, Grill E (2010) ABA perception and signaling. Trends Plant Sci 15:395–401
Reiser L, Modrusan Z, Margossian L et al (1995) The BELL1 gene encodes a homeodomain Âprotein involved in pattern formation in the Arabidopsis ovule primordium. Cell 83:735–742
Rhoades MW, Reinhart BJ, Lim LP, Burge CB, Bartel B, Bartel DP (2002) Prediction of plant microRNA targets. Cell 110:513–520
Sessa G, Carabelli M, Sassi M et al (2005) A dynamic balance between gene activation and repression regulates the shade avoidance response in Arabidopsis. Genes Dev 19:2811–2815
Shin D, Koo YD, Lee J et al (2004) Athb-12, a homeobox-leucine zipper domain protein from Arabidopsis thaliana, increases salt tolerance in yeast by regulating sodium exclusion. Biochem Biophys Res Commun 323:534–540
Shinozaki K, Yamaguchi-Shinozaki K, Seki M (2003) Regulatory network of gene expression in the drought and cold stress responses. Curr Opin Plant Biol 6:410–417
Simpson SD, Nakashima K, Narusaka Y, Seki M, Shinozaki K, Yamaguchi-Shinozaki K (2003) Two different novel cis-acting elements of erd1, a clpA homologous Arabidopsis gene, function in induction by dehydration stress and dark-induced senescence. Plant J 33:259–270
Smith HM, Boschke I, Hake S (2002) Selective interaction of plant homeodomain proteins mediates high DNA- binding affinity. Proc Natl Acad Sci USA 99:9579–9584
Soderman E, Mattsson J, Engstrom P (1996) The Arabidopsis homeobox gene ATHB-7 is induced by water deficit and by abscisic acid. Plant J 10:375–381
Soderman E, Hjellstrom M, Fahleson J, Engstrom P (1999) The HD-Zip gene ATHB6 in Arabidopsis is expressed in developing leaves, roots and carpels and up-regulated by water deficit conditions. Plant Mol Biol 40:1073–1083
Suzuki N, Sejima H, Tam R, Schlauch K, Mittler R (2011) Identification of the MBF1 heat-response regulon of Arabidopsis thaliana. Plant J 66:844–851
Talbert PB, Adler HT, Parks DW, Comai L (1995) The REVOLUTA gene is necessary for apical meristem development and for limiting cell divisions in the leaves and stems of Arabidopsis thaliana. Development 121:2723–2735
Tan QK, Irish VF (2006) The Arabidopsis zinc finger-homeodomain genes encode proteins with unique biochemical properties that are coordinately expressed during floral development. Plant Physiol 140:1095–1108
Tang G, Reinhart BJ, Bartel DP, Zamore PD (2003) A biochemical framework for RNA silencing in plants. Genes Dev 17:49–63
Tran LS, Nakashima K, Sakuma Y et al (2004) Isolation and functional analysis of Arabidopsis stress-inducible NAC transcription factors that bind to a drought-responsive cis-element in the early responsive to dehydration stress 1 promoter. Plant Cell 16:2481–2498
Tran LS, Nakashima K, Sakuma Y et al (2006) Co-expression of the stress-inducible zinc finger homeodomain ZFHD1 and NAC transcription factors enhances expression of the ERD1 gene in Arabidopsis. Plant J 49:46–63
Tron AE, Bertoncini CW, Chan RL, Gonzalez DH (2002) Redox regulation of plant homeodomain transcription factors. J Biol Chem 277:34800–34807
Uno Y, Furihata T, Abe H, Yoshida R, Shinozaki K, Yamaguchi-Shinozaki K (2000) Arabidopsis basic leucine zipper transcription factors involved in an abscisic acid-dependent signal transduction pathway under drought and high-salinity conditions. Proc Natl Acad Sci USA 97:11632–1163725
Urano K, Kurihara Y, Seki M, Shinozaki K (2010) ‘Omics’ analyses of regulatory networks in plant abiotic stress responses. Curr Opin Plant Biol 13:1–7
van der Graaff E, Laux T, Rensing SA (2009) The WUS homeobox-containing (WOX) protein family. Genome Biol 10:248.1–248.9
Vij S, Tyagi AK (2007) Emerging trends in the functional genomics of the abiotic stress response in crop plants. Plant Biotechnol J 5:361–380
Wang Y, Henriksson E, Soderman E, Henriksson KN, Sundberg E, Engstrom P (2003) The Arabidopsis homeobox gene, ATHB16, regulates leaf development and the sensitivity to photoperiod in Arabidopsis. Dev Biol 264:228–239
Yamaguchi-Shinozaki K, Shinozaki K (2006) Transcriptional regulatory networks in cellular responses and tolerance to dehydration and cold stresses. Annu Rev Plant Biol 57:781–803
Yu SW, Zhang LD, Zuo KJ, Tang DQ, Sun XF, Tang KX (2005) Brassica napus L. homeodomain leucine zipper gene BnHB6 responds to abiotic and biotic stresses. J Integr Plant Biol 47:1236–1248
Yu H, Chen X, Hong YY et al (2008) Activated expression of an Arabidopsis HD-START protein confers drought tolerance with improved root system and reduced stomatal density. Plant Cell 20:1134–1151
Zhang Y, Wu R, Qin G, Chen Z, Gu H, Qu LJ (2011) Over-expression of WOX1 leads to defects in meristem development and polyamine homeostasis in Arabidopsis. J Integr Plant Biol 53:493–506
Zhu JK (2002) Salt and drought stress signal transduction in plants. Annu Rev Plant Biol 53:247–273
Zhu J, Shi H, Lee BH et al (2004) An Arabidopsis homeodomain transcription factor gene, HOS9, mediates cold tolerance through a CBF-independent pathway. Proc Natl Acad Sci U S A 101:9873–9878
Acknowledgements
The work is supported financially by the Department of Science and Technology (grant file number SR/S0/PS/07/2011), Government of India, New Delhi and core grant from NIPGR. AB acknowledges the award of research fellowship from the Council of Scientific and Industrial Research, New Delhi.
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Bhattacharjee, A., Jain, M. (2013). Homeobox Genes as Potential Candidates for Crop Improvement Under Abiotic Stress. In: Tuteja, N., Singh Gill, S. (eds) Plant Acclimation to Environmental Stress. Springer, New York, NY. https://doi.org/10.1007/978-1-4614-5001-6_7
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