Kidney Function After In Vivo Gene Silencing of Uncoupling Protein-2 in Streptozotocin-Induced Diabetic Rats

  • Malou Friederich Persson
  • William J. Welch
  • Christopher S. Wilcox
  • Fredrik Palm
Conference paper
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 765)

Abstract

Kidney uncoupling protein 2 (UCP-2) increases in streptozotocin-induced diabetes, resulting in mitochondria uncoupling, i.e., increased oxygen consumption unrelated to active transport. The present study aimed to investigate the role of UCP-2 for normal and diabetic kidney function utilizing small interference RNA (siRNA) to reduce protein expression. Diabetic animals had increased glomerular filtration rate and kidney oxygen consumption, resulting in decreased oxygen tension and transported sodium per consumed oxygen. UCP-2 protein levels decreased 2 and 50% after UCP-2 siRNA administration in control and diabetic animals respectively. Kidney function was unaffected by in vivo siRNA-mediated gene silencing of UCP-2. The reason for the lack of effect of reducing UCP-2 is presently unknown but may involve compensatory mitochondrial uncoupling by the adenosine nucleotide transporter.

Keywords

Diabetes Kidney Mitochondria siRNA Uncoupling protein 2 

Notes

Acknowledgments

MPF and FP were supported by a grant from the Swedish Research Council and the Swedish Diabetes Foundation whereas WJW and CSW were supported by a grant from the NIH (DK 49870; DK 36079; HL 68686).

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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Malou Friederich Persson
    • 1
  • William J. Welch
    • 2
  • Christopher S. Wilcox
    • 2
  • Fredrik Palm
    • 1
    • 2
    • 3
  1. 1.Division of Integrative Physiology, Department of Medical Cell Biology, Biomedical CenterUppsala UniversityUppsalaSweden
  2. 2.Division of Nephrology and Hypertension, Department of Medicine, Medical CenterGeorgetown UniversityWashingtonUSA
  3. 3.Department of Medicine and Health SciencesLinköping UniversityLinköpingSweden

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