The RNA Recognition Motif and Messenger RNA
Messenger RNA in metazoans is regulated during and after transcription and during translation. In the nucleus as pre-mRNA, it is capped, spliced, and polyadenylated, then transported into the cytoplasm where it becomes a template for the ribosome. Proteins are responsible for much of the regulatory mechanisms involving pre-mRNA, and many of those proteins contain an RNA Recognition Motif (RRM) for binding RNA. Despite the common structure and conserved sequences of an RRM, this small globular domain is used for both specific and nonspecific binding to RNA. The RNA targets of an RRM range from a single nucleotide to eight nucleotides displayed in a hairpin loop; there is competition between RRMs for RNA-binding sites; there is exchange between proteins and interactions between RRMs. The story of how the RRM regulates mRNA is still being written.
KeywordsNuclear Magnetic Resonance Splice Site Internal Ribosome Entry Site Nuclear Magnetic Resonance Experiment Nuclear Magnetic Resonance Structure
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