The RNA Recognition Motif and Messenger RNA
Messenger RNA in metazoans is regulated during and after transcription and during translation. In the nucleus as pre-mRNA, it is capped, spliced, and polyadenylated, then transported into the cytoplasm where it becomes a template for the ribosome. Proteins are responsible for much of the regulatory mechanisms involving pre-mRNA, and many of those proteins contain an RNA Recognition Motif (RRM) for binding RNA. Despite the common structure and conserved sequences of an RRM, this small globular domain is used for both specific and nonspecific binding to RNA. The RNA targets of an RRM range from a single nucleotide to eight nucleotides displayed in a hairpin loop; there is competition between RRMs for RNA-binding sites; there is exchange between proteins and interactions between RRMs. The story of how the RRM regulates mRNA is still being written.
KeywordsTyrosine Amide Glycine Adenosine Electrophoresis
- Burd CG, Dreyfuss G (1994) Conserved structures and diversity of functions of RNA-binding proteins Science 265:615–621Google Scholar
- Katahira M, Miyanoiri Y, Enokizono Y, Matsuda G, Nagata T, Ishikawa F, Uesugi S (2001) Structure of the C-terminal RNA binding domain of hnRNP D0 (AUF1), its interactions with RNA and DNA, and change in backbone dynamics upon complex formation with DNA. J Mol Biol 311:973–988PubMedCrossRefGoogle Scholar
- Meisner NC, Filipowicz W (2010) Properties of the regulatory RNA binding protein HuR and its role in controlling miRNA repression. In: Grosshans H (ed) Regulation of microRNAs. Landes Bioscience and Springer Science, Austin/New YorkGoogle Scholar