Microdialysis in Antibiotic Research

  • Markus Zeitlinger
  • Sandrine Marchand
  • William Couet
  • Aline Barth
  • Hartmut Derendorf
Part of the AAPS Advances in the Pharmaceutical Sciences Series book series (AAPS, volume 4)


Traditionally, the PK of different anti-infective drugs is evaluated based on the plasma drug concentrations. However, in some cases those concentrations are not identical to those detected at the target site of the infection, i.e., in the affected tissue. In this context, microdialysis is a valuable technique applied for the quantification of free drug in the interstitial space fluid of a great variety of tissues. Considering that generally the infections are located in interstitial space fluid and only the free drug fraction is able to exert the anti-infective effect, it is clear that this approach has the necessary features to quantify the active fraction at the site of action. Using this information for consecutive pharmacokinetic/pharmacodynamic models is increasingly becoming state-of-the-art for optimizing dosing regimens of antibiotics.


Positron Emission Tomography High Performance Liquid Chromatography Minimal Inhibitory Concentration Epithelial Line Fluid Unbind Concentration 
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Copyright information

© American Association of Pharmaceutical Scientists 2013

Authors and Affiliations

  • Markus Zeitlinger
    • 1
  • Sandrine Marchand
    • 2
  • William Couet
    • 2
  • Aline Barth
    • 3
  • Hartmut Derendorf
    • 3
  1. 1.Department of Clinical PharmacologyMedical University of ViennaViennaAustria
  2. 2.School of Medicine and PharmacyUniversity of Poitiers, Pharmacologie des Anti-Infectieux, Pôle Biologie SantéPoitiers cedexFrance
  3. 3.Department of PharmaceuticsUniversity of Florida, College of PharmacyGainesvilleUSA

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