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High Volume Screening Procedures for Hypobetalipoproteinemic Activity in Rats

  • Paul E. Schurr
  • John R. Schultz
  • Charles E. Day
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 67)

Abstract

We describe high volume screening tests for hypobetalipoproteinemic agents in which compounds are administered orally to cholesterol-cholic acid fed (hypercholesterolemic) or normally fed weanling rats for 4 days. In these tests total serum cholesterol levels and heparin precipitating lipoproteins (HPL) are determined by automated analyses interfaced with a computer which eliminates all manual data reduction and provides necessary reports. The hypercholesterolemic rat test detects compounds which specifically reduce HPL (beta and pre beta lipoproteins) causing a decrease in the HPL:cholesterol ratio. Such activity is called hypobetalipoproteinemia. This activity is exhibited by bicyclo(2.2.2)-octyloxyaniline (U-26328) but not by any of the familiar hypocholesterolemic agents including clofibrate, lifibrate, nicotinic acid, probucol, triparanol, lentysine, D-thyroxine or the estrogens estrone and diethylstilbestrol.

Keywords

Nicotinic Acid Lower Density Lipoprotein Cholic Acid Screening Procedure Total Serum Cholesterol Level 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1976

Authors and Affiliations

  • Paul E. Schurr
    • 1
  • John R. Schultz
    • 1
  • Charles E. Day
    • 1
  1. 1.The Upjohn CompanyKalamazooUSA

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