Similarities and Dissimilarities between Outer Mitochondrial Membrane and Endoplasmic Reticulum

  • Gian Luigi Sottocasa
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 14)

Abstract

At the onset of this discussion it is important to define what I mean by endoplasmic reticulum and outer mitochondrial membrane. As most of biochemists I use the term endoplasmic reticulum as re-ferred to the microsomal fraction obtained by conventional differential centrifugation at 105,000 x g for one hour, after sedimentation of mitochondria. This fraction is heterogeneous and can be subfractionated by different means (1,2). The distribution of certain enzymes, however, can be regarded as sufficiently homogeneous throughout the different endoplasmic reticulum vescicles to be considered safe the use of such enzymes as markers of microsomes. These enzymes include glucose-6-phosphatase, NADPH cytochrome c reductase and a number of NADPH-linked functions of microsomes as well as cytochrome P450 (3). By outer mitochondrial membrane, on the other hand, I mean a fraction derived from isolated mitochondria, subjected to treatments capable to detach the outer from the inner membrane and subsequently fractionated either by continuous or discontinuous density-gradient as well as by differential centrifugation (3–12).

Keywords

Cholesterol Permeability Sucrose Magnesium Sedimentation 

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References

  1. 1.
    Dallner, G., Acta Pathol. et Microbiol. Scand. Suppl. 166: 1 (1963).Google Scholar
  2. 2.
    Dallman, P.R., Dallner, G., Bergstrand, A., and Ernster, L., J. Cell Biol. 41: 357 (1969).PubMedCrossRefGoogle Scholar
  3. 3.
    Sottocasa, G.L., Kuylenstierna, B., Ernster, L. and Bergstrand, A., J. Cell Biol. 32: 415 (1967).PubMedCrossRefGoogle Scholar
  4. 4.
    Sottocasa, G.L., Kuylenstierna, B., Ernster, L. and Bergstrand, A., in “Methods in Enzymology” (R.W. Estabrook and M.E. Pullman eds.), Vol. 10, p 448, Acad. Press, New York (1 967).Google Scholar
  5. 5.
    Sottocasa, G.L., Ernster, L., Kuylenstierna, B. and Bergstrand, A., in ‘Witochondrial Structure and Compartmentation“ (E. Quagliariello, S. Papa, E.C. Slater and J. M. Tager, eds.), p 74, Adriatica Editrice, Bari (1967).Google Scholar
  6. 6.
    Parsons, D.F., Williams, G.R. and Chance, B.,Ann. N. Y. Acad. Sci. 137: 643 (1966).CrossRefGoogle Scholar
  7. 7.
    Parsons, D.F., Williams, G.R., Thompson, W., Wilson, D. and Chance, B., in ‘Mitochondria) Structure and Compartmentation“ (E. Quagliariello, S. Papa, E.C. Slater and J.M. Tager, eds.), p 29, Adriatica Editrice, Bari (1967).Google Scholar
  8. 8.
    Parsons, D.F., and Williams, G.R., in “Methods in Enzymology” (R.W. Estabrook and M.E. Pullman, eds.), Vol. 10, p 443, Acad. Press, New York (1967).Google Scholar
  9. 9.
    Lèvy, M. and Sauner, M-T., Compt. Rend. Soc. Biol., 161: 277 (1967).Google Scholar
  10. 10.
    Lèvy, M., Toury, R. and Andrè, J., C. R. Acad. Sci. Paris, Sér. 6 263: 1766 ).Google Scholar
  11. 11.
    Schnaitman, C.A., Erwin, V.G. and Greenawalt, J.W., J. Cell Biol.,D, (19632: 719 (1967).Google Scholar
  12. 12.
    Schnaitman, C.A. and Greenawalt, J.W., J. Cell Biol., 8: 158 (1968).CrossRefGoogle Scholar
  13. 13.
    Ernster, L. and Kuylenstierna, B., in ‘Membranes of Mitochondria and Chloroplasts“ (E. Racker,ed.), p 172, Van Nostrand Reinhold Co., New York (1970).Google Scholar
  14. 14.
    Harding, B.W., Wong, S.H. and Nelson, D.H., Biochim. Biophys. Acta, 92: 415 (1964).Google Scholar
  15. 15.
    Omura, T., Sato, R., Cooper, D.Y., Rosenthal, 0. and Estabrook, R.W., Fed. Proc., 24: 1181 (1965).Google Scholar
  16. 16.
    Sottocasa, G.L. and Sandri, G., Abstr. Int. Symp. on Microsomes and Drug Oxidation, Tübingen (1969).Google Scholar
  17. 17.
    Sottocasa,G.L. and Sandri, G., Biochem. J., 116: 16p (1970).Google Scholar
  18. 18.
    Satre, M., Vignais, P.V. and Idelman, S., FEBS Letters, 5: 135 (1969).PubMedCrossRefGoogle Scholar
  19. 19.
    Stoffel, W. and Schiefer, H-G., Hoppe Seyler’s Z. Physiol. Chem., 349: 1017 (1968).CrossRefGoogle Scholar
  20. 20.
    Parsons, D.F. and Yano, Y., Biochim. Biophys. Acta, 135: 362 ( 1967CrossRefGoogle Scholar
  21. 21.
    Schnaitman, C.A., Proc. Nat. Acad. Sci., 63: 412 (1969).CrossRefGoogle Scholar
  22. 22.
    Sottocasa, G.L., in ‘Membrane Models and the Formation of Biological Membranes“ (L. Bolls and B.A. Pethica, eds.), p 229, North Holland Publ. Co., Amsterdam (1968).Google Scholar
  23. 23.
    Sottocasa, G.L. and Sandri,G., Abstr. 5th FEBS Meeting, Prague, p 131 (1968).Google Scholar
  24. 24.
    Sottocasa, G.L. and Sandri, G., in “Biochemistry of Intracellular Structures: Mitochondria and Endoplasmic Reticulum” (L. Wojtczak, W. Drabikowski and H. Strzelecka-Golaszewska, eds.), p 7, P. W. N. Warszawa (1969).Google Scholar
  25. 25.
    Ernster, L., Dallner, G. and Azzone, G.F., J. Biol. Chem., 238: 1124 (1963).Google Scholar
  26. 26.
    Kuylenstierna, B. and Sottocasa, G.L., umpublished data.Google Scholar
  27. 27.
    Strittmatter, P. and Velick, S.F., J. Biol. Chem., 228: 785 (1957).PubMedGoogle Scholar
  28. 28.
    Amons, R., Van den Bergh, S.G. and Slater, E.C., Biochim. Biophys. Acta, 162: 452 (1968).CrossRefGoogle Scholar
  29. 29.
    Sottocasa, G.L. and Sandri, G., It. J. Biochem., 17: 17 (1968).Google Scholar
  30. 30.
    Omura, T., Siekevitz, P. and Palade, G.E., J. Biol. Chem., 242: 2389 (1967).PubMedGoogle Scholar
  31. 31.
    Brunner, G. and Neupert, W., FEBS Letters, 1: 153 (1968).PubMedCrossRefGoogle Scholar
  32. 32.
    Bygrave, F.L. and Bucher, Th., European J. Biochem., 6: 256 (1968).CrossRefGoogle Scholar
  33. 33.
    Bygrave, F.L. and Bucher, Th., FEBS Letters, 1: 42 (1968).PubMedCrossRefGoogle Scholar
  34. 34.
    Bygrave, F.L., J. Biol. Chem., 244: 4768 ( 1969.PubMedGoogle Scholar
  35. 35.
    Beattie, D.S., Biochem. Biophys. Res. Commun., 35: 721 (1969).CrossRefGoogle Scholar
  36. 36.
    de Bernard, B., Getz, G.S. and Rabinowitz, M., Biochim. Biophys. Acta, 193: 58 (1969).CrossRefGoogle Scholar
  37. 37.
    Druyan, R., de Bernard, B. and Rabinowitz, M., J. Biol. Chem., 244: 5874 (1969).PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1971

Authors and Affiliations

  • Gian Luigi Sottocasa
    • 1
  1. 1.Istituto di Chimica BiologicaUniversità di TriesteTriesteItaly

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