The Subcellular Distribution of Human Placental Lactogen
In 1962 Josimovich and MacLaren (1) reported that the human placenta and retroplacental serum contain a lactogenic substance immunologically related to growth hormone. In the intervening years there have been many studies on the chemistry, biological effects, and secretion (2–7) of human placental lactogen (HPL) but comparatively little investigation on its intraplacental site of synthesis and storage. Sciarra (8) in an excellent review considered the evidence for the placental and cellular origin of HPL. He reported earlier that rabbit anti-HGH serum labeled with fluorescein isothiocyanate was limited to the syncytiotrophoblast layer of the villous epithelium (9). This communication describes the subcellular localization of HPL and outlines a simple method for its purification from fresh placentas.
KeywordsSucrose Starch Filtration Agar Hydroxide
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