Isolation of Collagen from Cortical Bovine Bone for Preparation of Porous Collagen Sponges
Fabrication of collagen sponges from type I collagen isolated from cortical bovine femur bone is reported. Collagen was extracted with 0.1 M EDTA by refreshing the solution every 24 h for 9 days. Sodium dodecyl sulfate poly-acrylamide gel electrophoresis (SDS-PAGE) demonstrated the isolation of intact pure type I collagen. The sponges were fabricated by freeze-drying and heat-dehydration cross-linking of collagen solution. The resulting sponges showed fibrils arranged in a highly porous network. These sponges can be used for tissue engineering as well as for bone-like composite fabrication.
KeywordsCollagen isolation Sponges Scaffolds Cortical bone
We thank Ryan Anderson of CalIT2, UCSD, for help in SEM imaging. Ana Castro acknowledges the University of California Institute for Mexico and the United States (UC MEXUS-CONACyT). This work has been founded by the Ceramics Program (Grant# DMR1006931).
- 5.Taira M, Sasaki K, Saitoh S, Nezu T, Araki Y (2006) Preparation of highly pore-interconnected porous collagen sponges using chloroform and ammonia. J Oral Tissue Eng 4(2):68–76Google Scholar
- 7.Dombi GW, Halsall HB (1985) Collagen fibril formation in the presence of sodium dodecyl sulphate. Biochem J 228:551–556Google Scholar