Modeling Single Cells in Systems Biology
One of the most powerful methods to study the dynamic behavior of protein networks is a single-cell analysis. Introduction of fluorescent proteins provided phenomenal approach to follow living cells in the spatiotemporal manner. In this chapter we shall discuss major principles and tools used in single-cell analysis of apoptotic cells. To understand why single-cell analysis is so important, we shall compare advantages and disadvantages of single-cell versus bulk measurements. Furthermore, we shall discuss the models based on the live cell imaging and information that can be obtained with these models. In particular, we shall focus on the studies devoted to the dynamics of caspase activation and mitochondrial outer membrane permeabilization.
KeywordsProtein Amount Live Cell Imaging Nuclear Export Signal Mitochondrial Outer Membrane Permeabilization Bulk Measurement
We acknowledge the Helmholtz Alliance on Systems Biology (NW1SBCancer) and Helmholtz-Russia Joint Research Groups-2008-2 for supporting our work.
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