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Approaches for the Identification and Characterization of RNA-Protein Interactions

  • Saiprasad Palusa
  • Jeffrey Wilusz
Chapter
Part of the Biophysics for the Life Sciences book series (BIOPHYS, volume 1)

Abstract

Based on analyses of conventional RNA binding motifs, there are well over 550 RNA binding proteins produced in living cells. Thus a detailed understanding of the mechanisms of post-transcriptional regulation requires an understanding of how these trans-acting factors act in both local and combinatorial fashions. The goal of this chapter is to provide a roadmap for approaching the identification of RNA–protein interactions and how to decipher fundamental aspects of their roles in the regulation of gene expression. Many of the techniques outlined below are applicable to the study of RNA–RNA interactions as well. Collectively they should provide a rational approach to gain mechanistic insights into a variety of post-transcriptional processes.

Keywords

Sodium Dodecyl Sulfate Cold Phosphate Buffer Saline Sepharose Bead Streptavidin Agarose TEDS Buffer 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgements

We wish to thank Ashley Neff, Stephanie Moon, Jerome Lee, John Anderson, and other members of the Wilusz laboratories for helpful comments and critical suggestions. Related RNA–protein work in the laboratory is supported by NIH grant GM072481 to J.W.

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Copyright information

© Springer Science+Business Media New York 2012

Authors and Affiliations

  1. 1.Department of Microbiology, Immunology and PathologyColorado State UniversityFort CollinsUSA

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