Probe Amplification Technologies

  • Fei Ye
  • Miao Cui
  • Tao Feng
  • Ivy Lee
  • Josephine Wu
  • Bingjiao Yin
  • David Zhang


Oligonucleotide probes provide a useful tool for the detection of target nucleic acids by the formation of a double-helical structure between complementary sequences. The stringent requirements of Watson–Crick base pairing make hybridization extremely specific. However, the detection of target sequence by hybridization is often insensitive due to the limited number of signal molecules that can be labeled on the probe. In general, the analytical sensitivity of probe hybridization is in the order of 106 molecules. Therefore, it cannot meet the needs of most clinical diagnostic applications. Many technologies have been developed to improve the detection sensitivity by amplifying the probe sequence bound to the target. All probe amplification technologies are developed based on the recent advancement in molecular biology and the understanding of in vivo nucleic acid synthesis, i.e., ligation, polymerization, transcription, digestion/cleavage, etc.


Fluorescence Resonance Energy Transfer Rolling Circle Amplification mecA Gene Invader Assay Rolling Circle Amplification Product 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Fei Ye
    • 1
  • Miao Cui
    • 1
  • Tao Feng
    • 1
  • Ivy Lee
    • 1
  • Josephine Wu
    • 1
  • Bingjiao Yin
    • 1
  • David Zhang
    • 1
  1. 1.Molecular Pathology Laboratory, Department of Pathology, Mount Sinai School of MedicineNew York UniversityNew YorkUSA

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