Sensors pp 481-485 | Cite as

Development of a pH Sensor with Integrated Reference Electrode for Cell Culture Monitoring

  • Andrea AdamiEmail author
  • Severino Pedrotti
  • Cristian Collini
  • Leandro Lorenzelli
Conference paper
Part of the Lecture Notes in Electrical Engineering book series (LNEE, volume 162)


This work presents the development of a “all-solid state” integrated reference electrode based on a PVC membrane doped with lipophilic salts (tetrabutylammonium tetrabutylborate (TBA-TBB) and Potassium tetrakis (4-chlorphenyl)borate (KTClpB)) for the integration with ISFETs sensors using a Si3N4 dielectric as sensitive material. The described system is intended for the implementation of fully integrated large sensor arrays for high throughput cell assays. Electrode preparation and evaluation of potential stability to pH is described, demonstrating the suitability of the approach.


Gate Dielectric Potential Stability Octyl Ether PEDOT Layer Fast Stabilisation 
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  1. 1.
    Ress C et al (2009) Development and characterization of a multiparametric microsensor for yeast cell growth monitoring. Proc Chem 1(1):1059–1062CrossRefGoogle Scholar
  2. 2.
    Simonis A et al (2004) New concepts of miniaturised reference electrodes in silicon technology for potentiometric sensor systems. Sens Actuator B 103:429–435CrossRefGoogle Scholar
  3. 3.
    Zhou J et al (2010) Fabrication of a microfluidic Ag/AgCl reference electrode and its application for portable and disposable electrochemical microchips. Electrophoresis 31:3083–3089CrossRefGoogle Scholar
  4. 4.
    Ivanova SA et al (2010) Development of miniature all-solid-state potentiometric sensing system. Sens Actuator B 146:199–205CrossRefGoogle Scholar
  5. 5.
    Tamburri E et al (2009) Growth mechanisms, morphology, and electroactivity of PEDOT layers produced by electrochemical routes in aqueous medium. Synthetic Met 159:406–414CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Andrea Adami
    • 1
    Email author
  • Severino Pedrotti
    • 1
  • Cristian Collini
    • 1
  • Leandro Lorenzelli
    • 1
  1. 1.FBK, Center for Materials and MicrosystemsTrentoItaly

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