Sensors pp 95-98 | Cite as

Hepcidin Detection by Affinity Based Sensing: A Possible Application in Clinical and Anti-doping Analysis

  • Simona Scarano
  • Ambra Vestri
  • Maria Laura Ermini
  • Maria Minunni
Conference paper
Part of the Lecture Notes in Electrical Engineering book series (LNEE, volume 162)

Abstract

The human Hepcidin-25 is a cysteine-rich proteinic hormone able to play a key role in systemic iron homeostasis. Indeed it can be associated with inflammatory events and iron dysfunctions but it may be even used as a new bio-marker to estimate indirectly erythropoietin blood doping. Affinity-based biosensors (ABBs) could be applied to hepcidin detection. In this study we adopted optical sensor system for label-free detection, based on Surface Plasmon Resonance (SPR), using as bioreceptor an anti human hepcidin-25 IgG. Limitations, advantages and potentials of this system are briefly discussed in light of behaviours and analytical performances detected.

Keywords

EDTA Hydroxide Carboxylate Immobilization Hydrochloride 

Notes

Acknowledgments

 Maria Minunni would like to thank the World Antidoping Agency (WADA), for funding within the “Scientific Research Grant-2010”, “Detection of Hepcidin As A New Biomarker of Erythropoiesis Stimulators Abuse: A Pilot Study”. We thank Dr. A. M. Bossi (University of Verona, Italy) and I. De Domenico (University of Utah, Internal Medicine Department, Hematology Division) for fruitful scientific discussions.

References

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Simona Scarano
    • 1
  • Ambra Vestri
    • 1
    • 2
  • Maria Laura Ermini
    • 1
  • Maria Minunni
    • 1
  1. 1.Dipartimento di ChimicaUniversità di FirenzeFirenzeItaly
  2. 2.Università degli Studi di Verona, Ca’ Vignal 1VeronaItaly

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