Hepcidin Detection by Affinity Based Sensing: A Possible Application in Clinical and Anti-doping Analysis
The human Hepcidin-25 is a cysteine-rich proteinic hormone able to play a key role in systemic iron homeostasis. Indeed it can be associated with inflammatory events and iron dysfunctions but it may be even used as a new bio-marker to estimate indirectly erythropoietin blood doping. Affinity-based biosensors (ABBs) could be applied to hepcidin detection. In this study we adopted optical sensor system for label-free detection, based on Surface Plasmon Resonance (SPR), using as bioreceptor an anti human hepcidin-25 IgG. Limitations, advantages and potentials of this system are briefly discussed in light of behaviours and analytical performances detected.
KeywordsEDTA Hydroxide Carboxylate Immobilization Hydrochloride
Maria Minunni would like to thank the World Antidoping Agency (WADA), for funding within the “Scientific Research Grant-2010”, “Detection of Hepcidin As A New Biomarker of Erythropoiesis Stimulators Abuse: A Pilot Study”. We thank Dr. A. M. Bossi (University of Verona, Italy) and I. De Domenico (University of Utah, Internal Medicine Department, Hematology Division) for fruitful scientific discussions.