Use of Matrix Assisted Laser Desorption/Ionization Imaging Mass Spectrometry (MALDI-IMS) in the Development of Novel Small Molecule Drugs

  • Jihai PangEmail author
  • Timothy L. Madden


Modern drug research and development includes the study of drug distribution and its metabolites within the biological organ and tissues. The high performance liquid chromatography coupled to a tandem mass spectrometer (HPLC-MS/MS) is currently widely employed in the study of drug metabolism and pharmacokinetic characterization of new drug molecules.

Today, the newest frontier, which adds the mass spectrometry to the arsenal of tools for the direct analysis of tissue biopsies and molecular diagnosis, is typically called Matrix Assisted Laser Desorption/Ionization Imaging Mass Spectrometry (MALDI-IMS). This powerful analytical tool is a new molecular imaging technique which in essence takes a mass spectral “snapshots” of intact tissue sections, revealing how the macromolecules, such as peptides and protein, and small molecular drugs and their metabolites are spatially distributed within a given biological tissue sample. In this chapter, MALDI-IMS technology is presented as well as applications of such technology in drug development and in clinical pharmacology. It provides direct, unambiguous evidence for the presence of drug substance in tissue sections.


Matrix Solution Image Mass Spectrometry Sinapinic Acid MALDI Matrix Matrix Selection 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.





Silver nanoparticles


Atmospheric pressure chemical ionization


Atmospheric pressure infrared MALDI mass ­spectrometry


α-Cyano-4-hydroxycinnamic acid


Desorption electrospray ionization


2,5-Dihydroxybenzoic acid


Electrospray ionization


High performance liquid chromatography - mass ­spectrometer


Laser ablation electrospray ionization mass spectrometry


Laser ablation-inductively coupled plasma-mass ­spectrometry


Laser desorption ionization


Laser desorption postionization




Matrix assisted laser desorption/ionization imaging mass spectrometry


MALDI-Fourier transform ion cyclotron resonance mass spectrometry


MALDI-Q-ion mobility-TOFMS


MALDI-Quadrupole-ion-trap-TOF mass spectrometry


MALDI-TOF-imaging mass spectrometry


MALDI Time-of –flight mass spectrometry


Magnetic resonance imaging


Matrix solution fixation




Nanoparticle-assisted laser desorption/ionization imaging mass spectrometry


Nanostructure initiator mass spectrometry


Oscillating capillary nebulizer




Prostate cancers


Sinapinic acid


Surface-assisted laser desorption/ionization mass ­spectrometry


Secondary ion mass spectrometry




Taurocholic acid


Taurochenodeoxycholic acid


Trifluoroacetatic acid


Thin layer chromatography


Ultraperformance liquid chromatography


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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  1. 1.Department of Experimental Therapeutics, Pharmaceutical Development CenterThe University of Texas MD Anderson Cancer CenterHoustonUSA

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