Internal Standards for Quantitative LC-MS Bioanalysis

  • Aimin TanEmail author
  • Nadine Boudreau
  • Ann Lévesque


Internal standards play critical roles in ensuring the accuracy of reported concentrations in LC-MS bioanalysis. How do you find an appropriate internal standard so that analyte losses and experimental variations during sample preparation, chromatographic separation, and mass spectrometric detection could be corrected? How is the concentration of an internal standard determined? Should internal standard responses be monitored during the analysis of incurred samples? What are the main causes for internal standard response variations? How do they impact the quantitation? Why are stable isotope labeled internal standards preferred? And yet one should still have an open-mind in their usage for the analysis of incurred samples. All these questions are addressed in this chapter supported by theoretical considerations and practical examples.


Quality Control Sample Mass Spectrometric Detection Deuterium Atom Deuterated Internal Standard Stable Isotope Label Internal Standard 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



We would like to thank Dr. Wen Jin in the University of Guelph for her reviewing of the draft manuscript and valuable comments. In addition, Tan would like to thank his family (Cailin and Joyce) for their support during the preparation of this book chapter.


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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  1. 1.BioPharma Services, Inc.TorontoCanada
  2. 2.PharmaNet Canada, Inc.QuébecCanada

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