Diagnostic Immunohistochemistry in Tumor Metastasis
Immunohistochemistry (IHC) refers to the process of localizing antigens (e.g. proteins) in cells of a tissue section using antibodies that bind specifically to these antigens in bisological tissues Ramos-Vara (Vet Pathol 42:405–426, 2005). Immunohistochemical staining is widely used in the diagnosis of abnormal cells such as those found in cancerous tumors and in metastasis Webster et al. (J Histochem Cytochem 57:753–761, 2009).
KeywordsEpidermal Growth Factor Receptor Gastrointestinal Stromal Tumor Epidermal Growth Factor Receptor Expression Surgical Pathologist Single Cell Clone
- Buchwalow IB, Böcker W (ed) (2010) Antibodies for immunohistochemistry. SpringerLink, p 153Google Scholar
- Ikeda S et al (2006) Combined immunohistochemistry of beta-catenin, cytokeratin 7, and cytokeratin 20 is useful in discriminating primary lung adenocarcinomas from metastatic colorectal cancer. BMC Cancer 6:31Google Scholar
- Riddle ND, Quigley BC, Browarsky I, Bui MM (2010) Leiomyosarcoma arising in the pancreatic duct: a case report and review of the current literature. Case Report Med 2010:252–364Google Scholar
- Sternberger LA, Hardy PH Jr, Cuculis JJ, Meyer HG (1970) The unlabeled antibody enzyme method of immunohistochemistry: preparation and properties of soluble antigen-antibody complex (horseradish peroxidase-antihorseradish peroxidase) and its use in identification of spirochetes. J Histochem Cytochem 18:315–333PubMedCrossRefGoogle Scholar