Abstract
There are several bacteriophages (phages) utilized for diagnosis of plague and pseudotuberculosis. Some of them could be used for antibacterial therapy of corresponding drug-resistant infections. Testing the ability of plague and pseudotuberculosis diagnostic phages to plate on Escherichia coli is important to estimate their diagnostic value and potential impact on normal microflora when using them as antibacterial drugs. In this work, 11 bacteriophages capable of lysing Yersinia pestis and/or Yersinia pseudotuberculosis were characterized including 6 plague and 3 pseudotuberculosis diagnostic phages. The efficiencies of phage plating were determined on Y. pestis, Y. pseudotuberculosis, and E. coli K-12 at both diagnostic (28°C) and body (37°C) temperatures. Only two phages could not form plaques on E. coli, Pokrovskaya, and φJA1. The rest of phages could plate on E. coli K-12 albeit with lower efficiencies at 28°C than on the corresponding Yersinia species. Efficiency of plating assays allowed us to determine the phages with both high diagnostic value and therapeutic potential including φA1122, L-413C, Pokrovskaya, φJA1, Y, T7Yp, and d’Herelle-m. Using site-directed mutagenesis, trans-complementation, and adsorption tests, we determined the E. coli K-12 receptors for three phages, L-413C, well-known enterobacteriophage P2, and PST in different sugar residues of the LPS outer core.
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Acknowledgements
We wish to thank Dr. Richard Calendar for critical reading of the manuscript and valuable comments, Drs Martin E Schriefer, Ian J Molineux, and Bakyt B Atshabar for providing bacteriophages. This research was supported by the Defense Threat Reduction Agency, Joint Science and Technology Office, Medical S&T Division. The findings and opinions expressed herein belong to the authors and do not necessarily reflect the official views of the WRAIR, the U.S. Army or the Department of Defense.
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Filippov, A.A., Sergueev, K.V., He, Y., Nikolich, M.P. (2012). Bacteriophages Capable of Lysing Yersinia pestis and Yersinia pseudotuberculosis: Efficiency of Plating Tests and Identification of Receptors in Escherichia coli K-12. In: de Almeida, A., Leal, N. (eds) Advances in Yersinia Research. Advances in Experimental Medicine and Biology, vol 954. Springer, New York, NY. https://doi.org/10.1007/978-1-4614-3561-7_16
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DOI: https://doi.org/10.1007/978-1-4614-3561-7_16
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