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Function of Collagen and Mast Cells in Acupuncture Points

  • Guanghong Ding
  • Di Zhang
  • Meng Huang
  • Lina Wang
  • Wei Yao
Chapter

Abstract

The acupoint is the responsive point that is stimulated during acupuncture treatment, and its structural basis and action mechanism have been largely elucidated by researchers. Clinical medical imaging and extensive studies on anatomy have indicated that the structural and functional basis for the human body’s meridians and acupoints is a complex system that primarily comprises connective tissues with numerous intertwining blood vessels, nerves, collagen fibers, and mast cells. Nevertheless, how the effective acupunctural signals transmit through acupoints to induce analgesia through a series of biochemical reactions along the meridians is a mystery. We have applied different acupuncture methods combined with morphology observations, mechanical signal detection, and animal pain threshold measurements to analyze the correlation between acupuncture analgesia and collagen fiber mechanical deformation as well as mast cell degranulation at acupoints. Furthermore, to explore the structural and functional basis of acupoints and the initiation of acupunctural signals, the patch clamp technique was used to investigate the dynamic characteristics of mechanosensitive proteins in relation to acupuncture at the cellular level. The results indicated that acupuncture analgesia was induced by physical stimulation at acupoints, where collagen fibers were deformed by stress and the surrounding mast cells were activated. The acupoint response is based on the spatial conformations of the blood vessels–mast cells–nerves. Once activated, mast cells are degranulated and release several types of bioactive substances, which increase blood vessel permeability. In addition, they act on the surrounding peripheral nerve endings, which induce a local peripheral nerve impulse and then transfer the acupunctural signal to the central nervous system. During this process, a positive feedback signal is generated by neurotransmitters acting on the mast cells and distributed along the meridians. An analgesic effect is produced and transmitted through an indirect effect on the organs and tissues along the meridians.

Keywords

Acupoint specificity Mast cells Connective tissue Acupuncture analgesia Signaling pathway Mechanical stimuli 

Notes

Acknowledgements

This work was supported by the National Basic Research Program of China (973 Program, Grant No. 2012CB518502), the National Natural Science Foundation of China (Grant No. 81102630), the Shanghai Leading Academic Discipline Project (Grant Nos. S30304, B112), the Science Foundation of Shanghai Municipal Commission of Science and Technology (Grant Nos. 09DZ1976600, 09dZ1974303, 10DZ1975800), and the Fudan Science Foundation for Young (Grant No. 09FQ07).

References

  1. Aumailley M, Gayraud B (1998) Structure and Biological Activity of the Extracellular Matrix. J Mol Med 76:(3–4): 253–265PubMedCrossRefGoogle Scholar
  2. Bergman MJ (2009) Assessing Adequate Treatment Response in Patients with Rheumatoid Arthritis. Clin Ther 31:(6): 1219–1231PubMedCrossRefGoogle Scholar
  3. Berman BM, Lao L, Langenberg P, Lee WL, Gilpin AM, Hochberg MC (2004) Effectiveness of Acupuncture as Adjunctive Therapy in Osteoarthritis of the Knee: A Randomized, Controlled Trial. Ann Intern Med 141:(12): 901–910PubMedGoogle Scholar
  4. Bienenstock J, Tomioka M, Matsuda H, Stead RH, Quinonez G, Simon GT, Coughlin MD, Denburg JA (1987) The Role of Mast Cells in Inflammatory Processes: Evidence for Nerve/Mast Cell Interactions. Int Arch Allergy Appl Immunol 82:(3–4): 238–243PubMedCrossRefGoogle Scholar
  5. Bourque CW, Oliet SH (1997) Osmoreceptors in the Central Nervous System. Annu Rev Physiol 59: 601–619PubMedCrossRefGoogle Scholar
  6. Brand DD, Kang AH, Rosloniec EF (2003) Immunopathogenesis of Collagen Arthritis. Springer Semin Immunopathol 25:(1): 3–18PubMedCrossRefGoogle Scholar
  7. Butterfield JH, Weiler D, Dewald G, Gleich GJ (1988) Establishment of an Immature Mast Cell Line from a Patient with Mast Cell Leukemia. Leuk Res 12:(4): 345–355PubMedCrossRefGoogle Scholar
  8. Chen EY, Shen XY, Dang RS, Chen HS, Cai DH, He WQ, Fei L (1998) A Relationship between Connecitve Tissue and Accumulation of Calcium with Points on Gb Channel Below Head. Shang Hai Zhen Jiu Za Zhi (Shanghai Journal of Acupuncture and Moxibustion) 17:(2): 36–37 (in Chinese with English abstract)Google Scholar
  9. Chen LW, Zhang BZ (1987) Discovery of the Neuromastocytic Junction on the Modelled Meridian Line in Mouse Skin– a Study by Light and Electron Microscopical Immunohistochemistry. Sheng Jing Jie Pou Xue Za Zhi (Chinese Journal of Neuroanatomy):(2): 253–258 (in Chinese with English Abstract)Google Scholar
  10. Cheng K, Shen XY, Ding GH, Wu F (2009) Relationship between Laser Acupuncture Analgesia and the Function of Mast Cells. Zhongguo Zhen Jiu (Chinese Acupuncture & Moxibustion) 29:(6): 478–483 (In Chinese with English abstract)Google Scholar
  11. Cho ZH, Hwang SC, Wong EK, Son YD, Kang CK, Park TS, Bai SJ, Kim YB, Lee YB, Sung KK, Lee BH, Shepp LA, Min KT (2006) Neural Substrates, Experimental Evidences and Functional Hypothesis of Acupuncture Mechanisms. Acta Neurol Scand 113:(6): 370–377PubMedCrossRefGoogle Scholar
  12. Colclasure JC, Holt JR (2003) Transduction and Adaptation in Sensory Hair Cells of the Mammalian Vestibular System. Gravit Space Biol Bull 16:(2): 61–70PubMedGoogle Scholar
  13. Coutaux A, Adam F, Willer JC, Le Bars D (2005) Hyperalgesia and Allodynia: Peripheral Mechanisms. Joint Bone Spine 72:(5): 359–371PubMedCrossRefGoogle Scholar
  14. Deng Y, Zeng T, Zhou Y, Guan X (1996) The Influence of Electroacupuncture on the Mast Cells in the Acupoints of the Stomach Meridian. Zhen Ci Yan Jiu (Acupuncuture Research) 21:(3): 68–70 (In Chinese with English abstract)Google Scholar
  15. Ding GH, Shen XY, Yao W, Dang RS, Yang J, Chen EY (2005) The Dynamic Mechanism of Directional Flow of Plasma and the Phenomena of Human Meridian. Zi Ran Ke Xue Jin Zhan (Progress in Natural Science) 11:(1): 811–818 (in Chinese)Google Scholar
  16. Ding GH, Yao W, Chu JH, Shen XY, Huang ZM, Liu H, Wang SZ, Yang J, Wei H, Fei L (2001) Spectral Characteristic of Infrared Radiations of Some Acupoint and Non-Acupoint Areas in Human Arm Surface. Ke Xue Tong Bao (Chinese Science Bulletin) 46:(23): 678–682 (in Chinese)Google Scholar
  17. Dirig DM, Salami A, Rathbun ML, Ozaki GT, Yaksh TL (1997) Characterization of Variables Defining Hindpaw Withdrawal Latency Evoked by Radiant Thermal Stimuli. J Neurosci Methods 76:(2): 183–191PubMedCrossRefGoogle Scholar
  18. Duan D, Winter C, Cowley S, Hume JR, Horowitz B (1997) Molecular Identification of a Volume-Regulated Chloride Channel. Nature 390:(6658): 417–421PubMedCrossRefGoogle Scholar
  19. Dvorak AM, Furitsu T, Ishizaka T (1993) Ultrastructural Morphology of Human Mast Cell Progenitors in Sequential Cocultures of Cord Blood Cells and Fibroblasts. Int Arch Allergy Immunol 100:(3): 219–229PubMedCrossRefGoogle Scholar
  20. Fei L, Chen HS, Cai DH, Yang SX, Xu JR, Chen EY, Dang RS, Ding GH, Shen XY, Tang Y (1998) Experimental Exploration and Research Prospect of Physical Base and Functional Characteristics of Meridian. Ke Xue Tong Bao (Chinese Science Bulletin) 43:(6): 658–672 (in Chinese)Google Scholar
  21. Gabriel SE, Crowson CS, O’Fallon WM (1999) The Epidemiology of Rheumatoid Arthritis in Rochester, Minnesota, 1955–1985. Arthritis Rheum 42:(3): 415–420PubMedCrossRefGoogle Scholar
  22. Galli SJ (1993) New Concepts About the Mast Cell. N Engl J Med 328:(4): 257–265PubMedCrossRefGoogle Scholar
  23. Galli SJ, Nakae S, Tsai M (2005) Mast Cells in the Development of Adaptive Immune Responses. Nat Immunol 6:(2): 135–142PubMedCrossRefGoogle Scholar
  24. Gao S, Ming C, Wu Y (1984) The Morphological Observation on Rat’s Peritoneal Mast Cells under Electroacupuncture at Point “Zuan San Li”. Zhen Ci Yan Jiu (Acupuncuture Research) 9:(1): 19–23 (in Chinese with English abstract)Google Scholar
  25. Guharay F, Sachs F (1984) Stretch-Activated Single Ion Channel Currents in Tissue-Cultured Embryonic Chick Skeletal Muscle. J Physiol 352: 685–701PubMedGoogle Scholar
  26. Hamill OP, Martinac B (2001) Molecular Basis of Mechanotransduction in Living Cells. Physiol Rev 81:(2): 685–740PubMedGoogle Scholar
  27. Han JS (2004) Acupuncture and Endorphins. Neurosci Lett 361:(1–3): 258–261PubMedCrossRefGoogle Scholar
  28. Harvima IT, Horsmanheimo L, Naukkarinen A, Horsmanheimo M (1994) Mast Cell Proteinases and Cytokines in Skin Inflammation. Arch Dermatol Res 287:(1): 61–67PubMedCrossRefGoogle Scholar
  29. Hathway GJ, Vega-Avelaira D, Moss A, Ingram R, Fitzgerald M (2009) Brief, Low Frequency Stimulation of Rat Peripheral C-Fibres Evokes Prolonged Microglial-Induced Central Sensitization in Adults but Not in Neonates. Pain 144:(1–2): 110–118PubMedCrossRefGoogle Scholar
  30. Hegyesi H, Darvas Z, Thurmond R, Falus A (2005). Histamine Receptors and Signaling(ed) Biophysical Aspects of Transmembrane Signaling, Springer Berlin Heidelberg. Berlin. 265–291Google Scholar
  31. Ho MW, Knight DP (1998) The Acupuncture System and the Liquid Crystalline Collagen Fibers of the Connective Tissues. Am J Chin Med 26:(3–4): 251–263PubMedCrossRefGoogle Scholar
  32. Hu JM, Ma WT, Tong H (2001). Acupuncture Researches in Last Decade, State Administration of Traditional Chinese Medicine of the People’s Republic of China. Beijing (in Chinese)Google Scholar
  33. Huang H, Zhan R, Yu XJ, Zhang D, Li WM, Ding GH (2009) Effects of Acupoint-Nerve Block on Mast Cell Activity, Manual Acupuncture- and Electroacupuncture-Induced Analgesia in Adjuvant Arthritis Rats. Zhen Ci Yan Jiu (Acupuncuture Research) 34:(1): 31–35, 56 (In Chinese with English abstract)Google Scholar
  34. Huang M, Xie YY, Ding GH (2010) Acupoint-Injection of Histamine Induced Analgesic Effect in Acute Adjuvant-Induced-Arthritis Rats. Zhen Ci Yan Jiu (Acupuncuture Research) 35:(2): 99–103 (In Chinese with English abstract)Google Scholar
  35. Irani AM, Craig SS, DeBlois G, Elson CO, Schechter NM, Schwartz LB (1987) Deficiency of the Tryptase-Positive, Chymase-Negative Mast Cell Type in Gastrointestinal Mucosa of Patients with Defective T Lymphocyte Function. J Immunol 138:(12): 4381–4386PubMedGoogle Scholar
  36. Itoh K, Katsumi Y, Hirota S, Kitakoji H (2007) Randomised Trial of Trigger Point Acupuncture Compared with Other Acupuncture for Treatment of Chronic Neck Pain. Complement Ther Med 15:(3): 172–179PubMedCrossRefGoogle Scholar
  37. Jia Y, Wang X, Varty L, Rizzo CA, Yang R, Correll CC, Phelps PT, Egan RW, Hey JA (2004) Functional Trpv4 Channels Are Expressed in Human Airway Smooth Muscle Cells. Am J Physiol Lung Cell Mol Physiol 287:(2): L272–278PubMedCrossRefGoogle Scholar
  38. Joe B, Griffiths MM, Remmers EF, Wilder RL (1999) Animal Models of Rheumatoid Arthritis and Related Inflammation. Curr Rheumatol Rep 1:(2): 139–148PubMedCrossRefGoogle Scholar
  39. Kimura M, Tohya K, Kuroiwa K, Oda H, Gorawski EC, Hua ZX, Toda S, Ohnishi M, Noguchi E (1992) Electron Microscopical and Immunohistochemical Studies on the Induction Of “Qi” Employing Needling Manipulation. Am J Chin Med 20:(1): 25–35PubMedCrossRefGoogle Scholar
  40. Kitamura Y (1989) Heterogeneity of Mast Cells and Phenotypic Change between Subpopulations. Annu Rev Immunol 7: 59–76PubMedCrossRefGoogle Scholar
  41. Kolodney MS, Wysolmerski RB (1992) Isometric Contraction by Fibroblasts and Endothelial Cells in Tissue Culture: A Quantitative Study. J Cell Biol 117:(1): 73–82PubMedCrossRefGoogle Scholar
  42. Koszegi Z, Kovacs P, Wilhelm M, Atlasz T, Babai N, Kallai V, Hernadi I (2006) The Application of in Vivo Microiontophoresis for the Investigation of Mast Cell-Neuron Interactions in the Rat Brain. J Biochem Biophys Methods 69:(1–2): 227–231PubMedCrossRefGoogle Scholar
  43. Langevin HM, Churchill DL, Cipolla MJ (2001) Mechanical Signaling through Connective Tissue: A Mechanism for the Therapeutic Effect of Acupuncture. Faseb J 15:(12): 2275–2282PubMedCrossRefGoogle Scholar
  44. Langevin HM, Yandow JA (2002) Relationship of Acupuncture Points and Meridians to Connective Tissue Planes. Anat Rec 269:(6): 257–265PubMedCrossRefGoogle Scholar
  45. Legler DF, Loetscher M, Jones SA, Dahinden CA, Arock M, Moser B (1996) Expression of High- and Low-Affinity Receptors for C3a on the Human Mast Cell Line, Hmc-1. Eur J Immunol 26:(4): 753–758PubMedCrossRefGoogle Scholar
  46. Lehoux S, Tedgui A (2004) Shear and Signal Transduction in the Endothelial Cell. Med Sci (Paris) 20:(5): 551–556 (in French)CrossRefGoogle Scholar
  47. Levi-Schaffer F, Shalit M (1993) Proliferation and Functional Responses of Bone Marrow-Derived Mast Cells after Activation. Cell Immunol 148:(2): 435–443PubMedCrossRefGoogle Scholar
  48. Liu JL, Chen ZR, Hu XL, Rong PJ (2000) Progresses in the Study on Meridian Theory in 2000. Zhen Ci Yan Jiu (Acupuncuture Research) 26:(1): 72–76 (in Chinese with English abstract)Google Scholar
  49. Lou XF, Mei J, Jiang SH, Shi Q, Zhang RF, Tang ML (2006) Study on Vascular Morphology of Integument Tissues of the Channel Area in the Leg. Zhongguo Zhen Jiu (Chinese Acupuncture & Moxibustion) 26:(9): 641–643 (In Chinese with English abstract)Google Scholar
  50. Maingret F, Patel AJ, Lesage F, Lazdunski M, Honore E (1999) Mechano- or Acid Stimulation, Two Interactive Modes of Activation of the Trek-1 Potassium Channel. J Biol Chem 274:(38): 26691–26696PubMedCrossRefGoogle Scholar
  51. Manheimer E, Linde K, Lao L, Bouter LM, Berman BM (2007) Meta-Analysis: Acupuncture for Osteoarthritis of the Knee. Ann Intern Med 146:(12): 868–877PubMedGoogle Scholar
  52. Manheimer E, White A, Berman B, Forys K, Ernst E (2005) Meta-Analysis: Acupuncture for Low Back Pain. Ann Intern Med 142:(8): 651–663PubMedGoogle Scholar
  53. Manheimer E, Zhang G, Udoff L, Haramati A, Langenberg P, Berman BM, Bouter LM (2008) Effects of Acupuncture on Rates of Pregnancy and Live Birth among Women Undergoing in Vitro Fertilisation: Systematic Review and Meta-Analysis. Bmj 336:(7643): 545–549PubMedCrossRefGoogle Scholar
  54. Masters DB, Domb AJ (1998) Liposphere Local Anesthetic Timed-Release for Perineural Site Application. Pharm Res 15:(7): 1038–1045PubMedCrossRefGoogle Scholar
  55. Metcalfe DD, Baram D, Mekori YA (1997) Mast Cells. Physiol Rev 77:(4): 1033–1079PubMedGoogle Scholar
  56. Mu X (1990) The Study of Meridian Substance -- Hyphothesis for Orderly Blood Capillary Network. Bei Jing Nong Xue Yuan Xue Bao (Journal of Beijing Agricultural College) 6:(1): 79–85 (in Chinese)Google Scholar
  57. Muraki K, Iwata Y, Katanosaka Y, Ito T, Ohya S, Shigekawa M, Imaizumi Y (2003) Trpv2 Is a Component of Osmotically Sensitive Cation Channels in Murine Aortic Myocytes. Circ Res 93:(9): 829–838PubMedCrossRefGoogle Scholar
  58. Nakamura F, Strittmatter SM (1996) P2y1 Purinergic Receptors in Sensory Neurons: Contribution to Touch-Induced Impulse Generation. Proc Natl Acad Sci U S A 93:(19): 10465–10470PubMedCrossRefGoogle Scholar
  59. Ndong C, Pradhan A, Puma C, Morello JP, Hoffert C, Groblewski T, O’Donnell D, Laird JM (2009) Role of Rat Sensory Neuron-Specific Receptor (Rsnsr1) in Inflammatory Pain: Contribution of Trpv1 to Snsr Signaling in the Pain Pathway. Pain 143:(1–2): 130–137PubMedCrossRefGoogle Scholar
  60. Nilsson G, Blom T, Kusche-Gullberg M, Kjellen L, Butterfield JH, Sundstrom C, Nilsson K, Hellman L (1994) Phenotypic Characterization of the Human Mast-Cell Line Hmc-1. Scand J Immunol 39:(5): 489–498PubMedCrossRefGoogle Scholar
  61. Pang X (1992) The Environment Conditions and Morphological Changes of Mast Cell Degranulation. Nei Meng Gu Yi Xue Yuan Xue Bao (Acta Academiae Medicinae Neimongol) 14:(1): 68–70 (in Chinese)Google Scholar
  62. Pierce SK, Politis AD (1990) Ca2(+)-Activated Cell Volume Recovery Mechanisms. Annu Rev Physiol 52: 27–42PubMedCrossRefGoogle Scholar
  63. Sandberg M, Lundeberg T, Lindberg LG, Gerdle B (2003) Effects of Acupuncture on Skin and Muscle Blood Flow in Healthy Subjects. Eur J Appl Physiol 90:(1–2): 114–119PubMedCrossRefGoogle Scholar
  64. Schwarz W, Rettinger, J., (2003). Foundations of Electrophysiology, Shaker Verl. Aachen, GermanyGoogle Scholar
  65. Shanahan F, Denburg JA, Fox J, Bienenstock J, Befus D (1985) Mast Cell Heterogeneity: Effects of Neuroenteric Peptides on Histamine Release. J Immunol 135:(2): 1331–1337PubMedGoogle Scholar
  66. Shen DK, Chen XT, Xu GS (1998) Effects of Acupuncture at the Lower Limb Section of Foot-Yangming Meridian on Stomach Physiology and Vascular Permeability. Zhong Guo Zhong Yi Ji Chu Yi Xue Za Zhi (Chinese Journal of Basic Medicine in Traditional Chinese Medicine) 4:(1): 52–54 (in Chinese)Google Scholar
  67. Shen J, Wenger N, Glaspy J, Hays RD, Albert PS, Choi C, Shekelle PG (2000) Electroacupuncture for Control of Myeloablative Chemotherapy-Induced Emesis: A Randomized Controlled Trial. Jama 284:(21): 2755–2761PubMedCrossRefGoogle Scholar
  68. Sillaber C, Bevec D, Butterfield JH, Heppner C, Valenta R, Scheiner O, Kraft D, Lechner K, Bettelheim P, Valent P (1993) Tumor Necrosis Factor Alpha and Interleukin-1 Beta Mrna Expression in Hmc-1 Cells: Differential Regulation of Gene Product Expression by Recombinant Interleukin-4. Exp Hematol 21:(9): 1271–1275PubMedGoogle Scholar
  69. Smith P, Mosscrop D, Davies S, Sloan P, Al-Ani Z (2007) The Efficacy of Acupuncture in the Treatment of Temporomandibular Joint Myofascial Pain: A Randomised Controlled Trial. J Dent 35:(3): 259–267PubMedCrossRefGoogle Scholar
  70. Song JM (1977) Mast Cells and Meridian Phenomenon. Liao Ning Zhong Yi Za Zhi (Liaoning Journal of Traditional Chinese Medicine) 19:(2): 59–61 (in Chinese)Google Scholar
  71. Staaf S, Oerther S, Lucas G, Mattsson JP, Ernfors P (2009) Differential Regulation of Trp Channels in a Rat Model of Neuropathic Pain. Pain 144:(1–2): 187–199PubMedCrossRefGoogle Scholar
  72. Sukharev S, Corey DP (2004) Mechanosensitive Channels: Multiplicity of Families and Gating Paradigms. Sci STKE 2004:(219): re4Google Scholar
  73. Theobald HE (2005) Dietary Calcium and Health. Nutrition Bulletin 30:(3): 237–277CrossRefGoogle Scholar
  74. Wang LN, Ding GH, Gu QB, Schwarz W (2009) Single-Channel Properties of a Stretch-Sensitive Chloride Channel in the Human Mast Cell Line Hmc-1. Eur Biophys J 39:(5): 757–767PubMedCrossRefGoogle Scholar
  75. Watanabe Y, Todome Y, Ohkuni H, Sakurada S, Ishikawa T, Yutsudo T, Fischetti VA, Zabriskie JB (2002) Cysteine Protease Activity and Histamine Release from the Human Mast Cell Line Hmc-1 Stimulated by Recombinant Streptococcal Pyrogenic Exotoxin B/Streptococcal Cysteine Protease. Infect Immun 70:(7): 3944–3947PubMedCrossRefGoogle Scholar
  76. Wedi B, Elsner J, Czech W, Butterfield JH, Kapp A (1996) Modulation of Intercellular Adhesion Molecule 1 (Icam-1) Expression on the Human Mast-Cell Line (Hmc)-1 by Inflammatory Mediators. Allergy 51:(10): 676–684PubMedCrossRefGoogle Scholar
  77. Witt C, Brinkhaus B, Jena S, Linde K, Streng A, Wagenpfeil S, Hummelsberger J, Walther HU, Melchart D, Willich SN (2005) Acupuncture in Patients with Osteoarthritis of the Knee: A Randomised Trial. Lancet 366:(9480): 136–143PubMedCrossRefGoogle Scholar
  78. Wu HG, Yao W, Zhou EH, Dong GR, Zuo CT, Liu HR (2007) Remarks on the Research of Specificities of Acupoints. Zhong Guo Zhen Jiu (Chinese Acupuncture and Moxibustion) 27:(1): 59–62 (In Chinese with English abstract)Google Scholar
  79. Xie H, He SH (2004) Research on Histamine Induced Mast Cell Activation. Zhong Hua Wei Sheng Wu He Mian Yi Xue Za Zhi (Chinese Journal of Microbiology and Immunology) 24:(3): 168 (in Chinese)Google Scholar
  80. Xu MH, Qiao YM, Dai JD (1989) The Tissue Structure of Acupuncture Point and Its Functional Basis. Shang Hai Zhen Jiu Za Zhi (Shanghai Journal of Acupuncture and Moxibustion) 8:(3): 28–30 (in Chinese)Google Scholar
  81. Xu XD, Cui HR, Lou XF, Jiang SH (2004) The Relation between Acupoint Shousanli and Radial Nerve & Vein. Zhen Jiu Lin Chuang Za Zhi (Journal of Clinical Acupuncture and Moxibustion) 20:(7): 47–48 (in Chinese)Google Scholar
  82. Xu ZX, Zheng LY (2003) Present Status of the Relationship between Meridians and Connective Tissues. Shang Hai Zhen Jiu Za Zhi (Shanghai Journal of Acupuncture and Moxibustion) 22:(3): 45–47 (in Chinese with English Abstract)Google Scholar
  83. Yamada K (1996) Relation between the Propagation Sensation Along Meridians and Lympatic Vessles. Guo Wai Yi Xue Zhong Yi Zhong Yao Fen Ce (Foreign Medical Sciences: volume of Traditional Chinese Medicine) 18:(2): 16–18 (in Chinese)Google Scholar
  84. Yan XH, Zhang XY, Liu CL, Dang RS, Huang YY, He W, Ding GH (2009) Do Acupuncture Points Exist? Phys Med Biol 54:(9): N143–150PubMedCrossRefGoogle Scholar
  85. Yang PC, Zheng PY, Wang CS (2005) Lipopolysaccharide Activates Human Mast Cells to Induce Intestinal Epithelial Barrier Dysfunction Int J Gastroenterology 4:(1): 112–114Google Scholar
  86. Yu XJ, Ding GH, Huang H, Lin J, Yao W, Zhan R (2009) Role of Collagen Fibers in Acupuncture Analgesia Therapy on Rats. Connect Tissue Res 50:(2): 110–120PubMedCrossRefGoogle Scholar
  87. Yuan L, Jiao PF, Tang L, Huang WH (2005) Anatomical Basic of Traditional Chinese Medical Channels. Zhong Guo Ji Chu Ke Xue (Chinese Basic Science) 7:(3): 5 (in Chinese)Google Scholar
  88. Zhang BZ, Wang JM (1985) Discovery of the Neuro-Mastocytic Junction on the Meridian Line in Human Skin. I.Efferent Neuromastocytic Junction. Shen Jing Jie Pou Xue Za Zhi (Chinese Journal of Neuronanatomy) 1:(1): 47–52 (in Chinese with English abstract)Google Scholar
  89. Zhang D, Ding GH, Shen XY, Yao W, Zhang Z, Zhang Y, Lin J, Gu QB (2008) Role of Mast Cells in Acupuncture Effect: A Pilot Study. Explore (NY) 4:(3): 170–177CrossRefGoogle Scholar
  90. Zhang D, Ding GH, Xue XY, Wang LN, Liu C (2004) Development of Researches on the Connective Tissues and Acupuncture. Zhen Ci Yan Jiu (Acupuncuture Research) 29: 77–81 (In Chinese with English abstract)Google Scholar
  91. Zhang D, Spielmann A, Ding GH, Schwarz W (2012) Activation of Mast-Cell Degranulation by Different Physical Stimuli Involves Activation of the Transient-Receptor-Protential Channel Trpv2. Phys Res 61:(1): 113–124Google Scholar
  92. Zhang D, Yao W, Ding GH, Yang J, Schwarz W, Fei Lun S, Liu F, Shen XY, Lao LY (2009) A Fluid Mechanics Model of Tissue Fluid Flow in Limb Connective Tissue--a Mechanism of Acupuncture Signal Transmission. Journal of Hydrodynamics, Ser. B 21:(5): 675–684Google Scholar
  93. Zhao QC (2000) Observation of Superficial Lymph Vessels at 12 Acupoint in Lower Limp. Zhong Yi Yao Xue Bao (Acta Chinese Medicine and pharmacology) 28:(2): 56–57 (in Chinese)Google Scholar
  94. Zhao Y, Shi WC, Sun QX, Zhang SH, Li XH, Jia J, Wang HS (2002) The Peripheral Neural Mechanism of the Sense of Propagation Along Meridian. Yi Xue Yan Jiu Tong Xun (Communication of Medical Research) 31:(12): 18–19 (in Chinese)Google Scholar
  95. Zhong AM, Wu JL, Hu YL (1994) Study on Correlation between the Mast Cell and the Acupoint. World J Acupunc Mox 4:(4): 53–59Google Scholar
  96. Zhu Z, Xu R (1990) Morphometric Observation on the Mast Cells under the Acupuncture Meridian Lines. Zhen Ci Yan Jiu (Acupuncture Research) 15:(2): 157–158 (In Chinese with English abstract)Google Scholar
  97. Zou YH, He ZM, Tang JH, Zhong TJ, Zeng YL (1993) Effect of the Acupuncture of the Twirling Reinforcing- Reducing Method on Rat’s Mast Cells in Transparent Fenestra of Mesentery. Guang Dong Jie Pou Xue Tong Bao (Guang Dong Bulletin of Anatomy) 15:(2): 107–111 (in Chinese with English abstract)Google Scholar

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© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Guanghong Ding
    • 1
    • 2
  • Di Zhang
    • 1
    • 2
  • Meng Huang
    • 1
  • Lina Wang
    • 3
  • Wei Yao
    • 1
  1. 1.Department of Mechanics and Engineering ScienceFudan UniversityShanghaiChina
  2. 2.Shanghai Research Center for Acupuncture and MeridiansFudan UniversityShanghaiChina
  3. 3.College of Acupuncture and MoxibustionShanghai University of Traditional Chinese MedicineShanghaiChina

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