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Role of Sperm–Hyaluronic Acid Binding in the Evaluation and Treatment of Subfertile Men with ROS-Affected Semen: Assessment of Sperm with Oxidative Damage and HA-Mediated ICSI Sperm Selection

  • Ciler Celik-OzenciEmail author
  • Gabor Huszar
Chapter

Abstract

Excessive levels of free radicals diminish the functional integrity of spermatozoa. Levels of reactive oxygen species (ROS) produced by spermatozoa were negatively correlated with the quality of sperm in the original semen. “Intrinsic,” “extrinsic,” and “iatrogenic” sources of ROS production were identified within semen: Intrinsic sources of ROS in semen are morphologically abnormal and arrested maturity spermatozoa and leukocytes. Poor sperm quality showing attributes of arrested sperm maturation is linked to increased ROS generation as a consequence of excess residual cytoplasm related to the arrest of cytoplasmic extrusion in terminal spermiogenesis. In the past 20 years, the Huszar lab has studied several key events of sperm maturation, including cytoplasmic extrusion and expression of the HspA2 chaperone protein. In experiments related to sperm function and fertilizing potential, Huszar et al. have established that, simultaneously with cytoplasmic extrusion during terminal spermiogenesis, there is a remodeling of the plasma membrane that facilitates the formation of the zona pellucida- and hyaluronic acid (HA)-binding sites. The studies with HA immobilized to glass slides or Petri dishes showed that sperm firmly bind to HA. However, not all sperm exhibited HA binding ability. These data supported the hypothesis that the ability of sperm–HA binding is related to sperm cellular maturity.

Keywords

Sperm–hyaluronic acid binding Sperm function Oxidative stress Reactive oxygen ­species Male infertility Intracytoplasmic sperm selection DNA chain degradation 

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Further Reading

  1. Gomez E, Buckingham DW, Brindle J, et al. Development of an image analysis system to monitor the retention of residual cytoplasm by human spermatozoa: correlation with biochemical markers of the cytoplasmic space, oxidative stress, and sperm function. J Androl. 1996;17:276–87.PubMedGoogle Scholar
  2. Pasqualotto FF, Sharma RK, Kobayashi H, et al. Oxidative stress in normospermic men undergoing infertility evaluation. J Androl. 2001;22:316–22.PubMedGoogle Scholar
  3. Garrido N, Meseguer M, Alvarez J, et al. Relationship among standard semen parameters, glutathione peroxidase/glutathione reductase activity, and mRNA expression and reduced glutathione content in ejaculated spermatozoa from fertile and infertile men. Fertil Steril. 2004;82:1059–66.PubMedGoogle Scholar
  4. Said TM, Agarwal A, Sharma RK, et al. Human sperm superoxide anion generation and correlation with semen quality in patients with male infertility. Fertil Steril. 2004;82:871–7.PubMedGoogle Scholar
  5. Said TM, Agarwal A, Sharma RK, et al. Impact of sperm morphology on DNA damage caused by oxidative stress induced by beta-nicotinamide adenine dinucleotide phosphate. Fertil Steril. 2005;83: 95–103.PubMedGoogle Scholar
  6. Agarwal A, Sharma RK, Nallella KP, et al. Reactive oxygen species as an independent marker of male factor infertility. Fertil Steril. 2006;86:878–85.PubMedGoogle Scholar
  7. Sakkas D, Alvarez JG. Sperm DNA fragmentation: mechanism of origin, impact onreproductive outcome, and analysis. Fertil Steril. 2010; 93(4): 1027–1036.PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  1. 1.Department of Histology and Embryology, School of MedicineAkdeniz UniversityAntalyaTurkey
  2. 2.Sperm Physiology LaboratoryYale School of MedicineNew HavenUSA

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