Detection technologies developed in the last 10 years have been directed to find more rapid, specific, and sensitive methods to detect multiple targets simultaneously. Much emphasis has been put on the desired characteristics of high sensitivity, high specificity, and cost-effectiveness, which are of great importance in routine high-volume food diagnostics. Developments in microfluidics, microarray technology, and nanotechnology facilitate the development of novel detection platforms such as protein and polysaccharide microarrays, lab on a chip, biosensors, and high-throughput sequencing technologies. Such methods will be used for larger applications and will open new avenues in food microbiology. These multiplexed assays will allow the development of tests for the detection of virtually any combination of target sequences in any type of sample that contains nucleic acid material. Genomics, proteomics, and other areas of study concerning microbial cells will provide new opportunities for achieving objectives that were hard to imagine not so long ago. These promising new technologies are making the transition from the research laboratory to routine diagnostic use. Obviously, the new technologies will also need to be carefully evaluated and thoroughly validated for specific applications.
KeywordsProtein microarrays Polysaccharide microarrays Phenotype microarrays Biosensors Nanotechnology Next-generation high-throughput sequencing technologies
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