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Detection, Identification, and Analysis of Foodborne Pathogens

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Book cover Microarray Detection and Characterization of Bacterial Foodborne Pathogens

Part of the book series: SpringerBriefs in Food, Health, and Nutrition ((BRIEFSFOOD))

Abstract

The detection and enumeration of microorganisms in food are an essential part of any quality control or food safety plan. Traditional methods of detecting foodborne pathogenic bacteria are often time-consuming because of the need for growth in culture media, followed by isolation, biochemical and/or serological identification, and in some cases, subspecific characterization. Advances in technology have made detection and identification faster, more sensitive, more specific, and more convenient than traditional assays. These new methods include for the most part antibody- and DNA-based tests, and modifications of conventional tests made to speed up analysis and reduce handling. With few exceptions, almost all assays used to detect specific pathogens in foods are qualitative assays, as they still lack sufficient sensitivity for direct testing and require some growth in an enrichment medium before analysis. One of the most challenging problems to circumvent with these assays is sample preparation. The possibilities of combining different rapid methods, including improved technologies for separation and concentration of specific bacteria, and for DNA extraction and purification, will facilitate the direct detection of pathogens in food. The goal is to avoid the enrichment, providing rapid alternatives to conventional quantitative culture methods. Further improvements, especially in genetic methods, can be expected, including the use of DNA microarray technology.

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Authors and Affiliations

  1. Department of Bioinformatics and Public Health, Spanish National Institute of Health ‘Carlos III’, Majadahonda, Madrid, Spain

    Guillermo López-Campos & Victoria López-Alonso

  2. Department of Food Technology, Spanish National Institute for Agricultural and Food Research and Technology (INIA), Madrid, Spain

    Joaquín V. Martínez-Suárez

  3. Department of Animal Health, Complutense University, Madrid, Spain

    Mónica Aguado-Urda

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  1. Guillermo López-Campos
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  2. Joaquín V. Martínez-Suárez
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  3. Mónica Aguado-Urda
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  4. Victoria López-Alonso
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© 2012 Guillermo Lopez-Campos; Joaquin V. Martinez-Suarez; Mónica Aguado-Urda; Victoria Lopez Alonso

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López-Campos, G., Martínez-Suárez, J.V., Aguado-Urda, M., López-Alonso, V. (2012). Detection, Identification, and Analysis of Foodborne Pathogens. In: Microarray Detection and Characterization of Bacterial Foodborne Pathogens. SpringerBriefs in Food, Health, and Nutrition. Springer, Boston, MA. https://doi.org/10.1007/978-1-4614-3250-0_2

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