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Scanning Electron Microscopy for Fungal Sample Examination

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Laboratory Protocols in Fungal Biology

Part of the book series: Fungal Biology ((FUNGBIO))

Abstract

This chapter presents general and specific methods for preparing fungi, plant, and seed tissues infected with fungi, and nematodes parasited by fungi using scanning electron microscopy (SEM). Conventional methods with chemical fixation as well as cryofixation are included, with details for subsequent steps of sample preparation to get optimal fungal specimens for visualization and examination through SEM.

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Acknowledgements

The authors gratefully acknowledge CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico), CAPES (Coordenadoria de Aperfeiçoamento de Pessoal de Nível Superior) and FAPEMIG (Fundação de Amparo à Pesquisa do Estado de Minas Gerais—Brazil) for financial support to Ultra-structural analysis and electron microscopy laboratory of the Ufla, Brazil.

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Authors and Affiliations

  1. Department of Phytopathology, Ultra-structural Analysis and Electron Microscopy Laboratory—Ufla, Lavras, Minas Gerais, 37200-000, Brazil

    Eduardo Alves & Gilvaine Ciavareli Lucas

  2. Departamento de Fitopatologia, Universidade Federal de Lavras, Caixa postal 3037, Lavras, Minas Gerais, 37200-000, Brazil

    Gilvaine Ciavareli Lucas & Edson Ampélio Pozza

  3. Department of Soil and Rural Engineering, Campus of the Federal University of Mato Grosso, Av. Fernando Correa da Costa, 2367, Boa Esperança, Cuiaba, Mato Grosso, 78060900, Brazil

    Marcelo de Carvalho Alves

Authors
  1. Eduardo Alves
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  2. Gilvaine Ciavareli Lucas
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  3. Edson Ampélio Pozza
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  4. Marcelo de Carvalho Alves
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Corresponding author

Correspondence to Marcelo de Carvalho Alves .

Editor information

Editors and Affiliations

  1. , Department of Biochemistry, National University of Ireland Galway, University Road, Galway, N/A, Ireland

    Vijai Kumar Gupta

  2. , Department of Biochemistry, National University of Ireland Galway, University Road, Galway, N/A, Ireland

    Maria G. Tuohy

  3. , Department of Biochemistry, National University of Ireland Galway, University Road, Galway, N/A, Ireland

    Manimaran Ayyachamy

  4. , Manufacturing Sciences and Technology, Pfizer Ireland Pharmaceuticals, The Pfizer Biotech Campus at Grange Castle, Clondalkin, N/A, Dublin, Ireland

    Kevin M. Turner

  5. , School of Natural Sciences, National University of Ireland Galway, University Road, Galway, N/A, Ireland

    Anthonia O’Donovan

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Alves, E., Lucas, G.C., Pozza, E.A., de Carvalho Alves, M. (2013). Scanning Electron Microscopy for Fungal Sample Examination. In: Gupta, V., Tuohy, M., Ayyachamy, M., Turner, K., O’Donovan, A. (eds) Laboratory Protocols in Fungal Biology. Fungal Biology. Springer, New York, NY. https://doi.org/10.1007/978-1-4614-2356-0_8

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