Abstract
It is challenging to prepare protoplasts and establish a transformation system in any toxin that produces slow-growing fungus, like Undifilum oxytropis. This chapter describes a protocol to generate a nontransient transformation system with the help of introducing a foreign heterologous gene to a toxin producing slow-growing fungi. This system will be useful to study genes in metabolically important pathways.
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References
Akamatsu H, Itoh Y, Otani H, Kohmoto K (1997) AAL-toxin-deficient mutants of Alternaria alternata tomato pathotype by restriction enzyme-mediated-integration. Phytopathology 87:967–972
Fierro F, Gutierrez S, Diez B, Martin JF (1993) Resolution of four large chromosomes in penicillin-producing filamentous fungi: the penicillin gene cluster is located on chromosome II (9.6 Mb) in Penicillium notatum and chromosome I (10.4 Mb) in Penicillium chrysogenum. Mol Gen Genet 241:573–578
Panaccione DG, Johnson RD, Wang J, Young CA, Damrongkool P, Scott B et al (2001) Elimination of ergovaline from a grass-Neotyphodium endophyte symbiosis by genetic modification of the endophyte. Proc Natl Acad Sci U S A 98:12820–12825
Pryor B, Creamer R, Shoemaker R, McLain-Romero J, Hambleton S (2009) Undifilum, a new genus for endophytic Embellisia oxytropis and parasitic Helminthosporium bornmuelleri on legumes. Botany 87:178–194
Braun K, Romero J, Liddell C, Creamer R (2003) Production of swainsonine by fungal endophytes of locoweed. Mycol Res 107:980–988
Harris CM, Schneider MJ, Ungemach FS, Hill JE, Harris TM (1988) Biosynthesis of the toxic indolizidine alkaloids slaframine and swainsonine in Rhizoctonia leguminicola: metabolism of 1-hydroxyindolizidines. J Am Chem Soc 110:940–949
Ralphs MH, Gardner DR, Turner DL, Pfister JA, Thacker E (2002) Predicting toxicity of tall larkspur (Delphinium barbeyi): measurement of the variation in alkaloid concentration among plants and among years. J Chem Ecol 28:2327–2341
Mukherjee S, Dawe AL, Creamer R (2010) Development of a transformation system in the Âswainsonine producing, slow growing endophytic Âfungus, Undifilum oxytropis. J Microbiol Methods 81:160–165
Ralphs MH, Creamer R, Baucom D, Gardner DR, Welsh SL, Graham JD et al (2008) Relationship between the endophyte Embellisia spp. and the toxic alkaloid swainsonine in major locoweed species (Astragalus and Oxytropis). J Chem Ecol 34:32–38
Suzuki N, Geletka LM, Nuss DL (2000) Essential and dispensable virus-encoded replication elements revealed by efforts To develop hypoviruses as gene expression vectors. J Virol 74:7568–7577
Acknowledgements
The authors would like to thank Dr. Richard Richins, Dr. Soum Sanogo, Dr. Swati Mukherjee, and Deana Beacom of New Mexico State University for technical assistance and constructive discussion.
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Mukherjee, S., Creamer, R. (2013). Transformation Methods for Slow-Growing Fungi. In: Gupta, V., Tuohy, M., Ayyachamy, M., Turner, K., O’Donovan, A. (eds) Laboratory Protocols in Fungal Biology. Fungal Biology. Springer, New York, NY. https://doi.org/10.1007/978-1-4614-2356-0_43
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DOI: https://doi.org/10.1007/978-1-4614-2356-0_43
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