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Detection and Identification of Fungal Microbial Volatile Organic Compounds by HS-SPME-GC–MS

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Book cover Laboratory Protocols in Fungal Biology

Part of the book series: Fungal Biology ((FUNGBIO))

Abstract

A method based on solid phase microextraction coupled to gas chromatography–mass spectrometry (GC–MS) for the detection and identification of microbial volatile organic compounds (MVOCs) in the headspace of filamentous fungi is presented. MVOCs are identified by comparison of mass spectra and linear temperature programmed retention indices (LTPRIs) with database entries and LTPRIs published in literature. The presented method enables the monitoring of the formation of volatile metabolites for defined time intervals during cultivation of the investigated fungus. The experimental procedure is exemplified with Fusarium graminearum and Trichoderma atroviride but can also be used to detect, identify and profile MVOCs produced by other filamentous fungi.

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Acknowledgement

The financial support by the Austrian Science Fund (FWF projects F3702 and F3706) is gratefully acknowledged.

Author information

Authors and Affiliations

  1. Department for Agrobiotechnology (IFA-Tulln), University of Natural Resources and Life Sciences Vienna, Konrad Lorenz Str. 20, Tulln, 3430, Austria

    Bernhard Kluger, Denise Schöfbeck & Rainer Schuhmacher

  2. Research Area Gene Technology and Applied Biochemistry, Institute for Chemical Engineering, Vienna University of Technology, Getreidemarkt 9, Vienna, 1060, Austria

    Susanne Zeilinger

  3. Institute of Applied Genetics and Cell Biology, University of Natural Resources and Life Sciences Vienna, Konrad Lorenz Str. 24, Tulln, 3430, Austria

    Gerlinde Wiesenberger

Authors
  1. Bernhard Kluger
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  2. Susanne Zeilinger
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  3. Gerlinde Wiesenberger
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  4. Denise Schöfbeck
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  5. Rainer Schuhmacher
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Editor information

Editors and Affiliations

  1. , Department of Biochemistry, National University of Ireland Galway, University Road, Galway, N/A, Ireland

    Vijai Kumar Gupta

  2. , Department of Biochemistry, National University of Ireland Galway, University Road, Galway, N/A, Ireland

    Maria G. Tuohy

  3. , Department of Biochemistry, National University of Ireland Galway, University Road, Galway, N/A, Ireland

    Manimaran Ayyachamy

  4. , Manufacturing Sciences and Technology, Pfizer Ireland Pharmaceuticals, The Pfizer Biotech Campus at Grange Castle, Clondalkin, N/A, Dublin, Ireland

    Kevin M. Turner

  5. , School of Natural Sciences, National University of Ireland Galway, University Road, Galway, N/A, Ireland

    Anthonia O’Donovan

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Kluger, B., Zeilinger, S., Wiesenberger, G., Schöfbeck, D., Schuhmacher, R. (2013). Detection and Identification of Fungal Microbial Volatile Organic Compounds by HS-SPME-GC–MS. In: Gupta, V., Tuohy, M., Ayyachamy, M., Turner, K., O’Donovan, A. (eds) Laboratory Protocols in Fungal Biology. Fungal Biology. Springer, New York, NY. https://doi.org/10.1007/978-1-4614-2356-0_42

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