Abstract
Because of breakage of rigid fungal cell walls, the major challenge for DNA isolation from fungi is to obtain samples of good quality and quantity. We developed a fast and efficient DNA isolation method from fungi that was successfully applied to Fusarium spp., Colletotrichum spp., Trichoderma spp., Gliocladium roseum, and Lasiodiplodia theobromae.
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References
Raeder U, Broda P (1985) Rapid preparation of DNA from filamentous fungi. Appl Microbiol 1:17–20
Cubeta MA, Echandi E, Abernethy T, Vilgalys R (1991) Characterization of anastomosis groups of binucleate Rhizoctonia species using restriction analysis of an amplified ribosomal RNA. Phytopathology 81:1395–1400
Clarke DL, Woodlee GL, McClelland CM, Seymour TS, Wickes BL (2001) The Cryptococcus neoformans STE11alpha gene is similar to other fungal mitogen-activated protein kinase kinase kinase (MAPKKK) genes but is mating type specific. Mol Microbiol 40:200–213
Graham GC, Mayers P, Henry RJ (1994) A simplified method for the preparation of fungal genomic DNA for PCR and RAPD analysis. Biotechniques 16:48–50
Min J, Arganoza MT, Ohrenberger J, Xu C, Akins RA (1995) Alternative methods of preparing whole-cell DNA from fungi for dot-blot, restriction analysis, and colony filter hybridization. Anal Biochem 225:94–100
Gupta VK (2009) Molecular characterization of Fusarium wilt pathogens of guava (Psidium guajava L.) using RAPD and microsatellite maker. Ph.D. Thesis. Dr. R.M.L. Avadh University, Faizabad, Uttar Pradesh, India
Gupta VK (2012) Genetic Diversity of Fusarium wilt pathogens of Guava in India. J Environ Sci Health [B] 47:315–325
Gupta VK (2012) Characterization of virulence gene loci in the genome of Fusarium spp. associated with wilt disease of guava in India. Arch Phytopathol Plant Protect (accepted), APPP ID: 45(2):244–259
Acknowledgements
The authors are very thankful to Head, Department of Microbiology, R. M. L. Avadh University, Faizabad, UP, India, and Prof. Shakti Baijal, Ex-Dean, FASC, MITS University, Rajasthan, for providing the necessary research grants.
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Gupta, V.K., Tuohy, M.G., Gaur, R. (2013). Methods for High-Quality DNA Extraction from Fungi. In: Gupta, V., Tuohy, M., Ayyachamy, M., Turner, K., O’Donovan, A. (eds) Laboratory Protocols in Fungal Biology. Fungal Biology. Springer, New York, NY. https://doi.org/10.1007/978-1-4614-2356-0_36
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DOI: https://doi.org/10.1007/978-1-4614-2356-0_36
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