Abstract
Traditional methods for detecting proteases in fungi require the separation of product from substrate. These methods are time-consuming, laborious, and not amenable to high-throughput analysis. A simple alternative method is described here that utilizes Coomassie dye reagent to follow the time-dependent proteolytic loss of a macromolecular protein substrate.
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O’Donoghue, A.J., Mahon, C.S. (2013). Detection and Quantification of Endoprotease Activity Using a Coomassie Dye-Binding Assay. In: Gupta, V., Tuohy, M., Ayyachamy, M., Turner, K., O’Donovan, A. (eds) Laboratory Protocols in Fungal Biology. Fungal Biology. Springer, New York, NY. https://doi.org/10.1007/978-1-4614-2356-0_25
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DOI: https://doi.org/10.1007/978-1-4614-2356-0_25
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