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Nanorobotic Manipulation and Sensing for Biomedical Applications

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Abstract

We developed a method for manipulating a single virus (size: 100nm) that employs optical tweezers in conjunction with dielectrophoretic (DEP) concentration of viruses on a microfluidic chip. The DEP force in the sample chamber concentrates the virus and prevents it from adhering to the glass substrate. The concentrated virus is transported to the sample selection section where it is trapped by optical tweezers. We demonstrated concentration of the virus using the DEP force, transported a single virus, and made it contact to a specific H292 cell. We also developed local measurement with functional gel-microtool for cell measurement. Gel-microtool was impregnated with indicators. In this chapter, bromothymol blue (BTB) and bromocresol green (BCG) were employed as pH indicators. Rhodamine B is temperature-sensitive fluorescent dye and is used for temperature measurement. Gel-microtool is manipulated by optical tweezers. Measurement is performed by detecting the color and the fluorescence intensity of each gel-microtool. We succeeded in pH measurement and temperature measurement using the gel-microtools.

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Authors and Affiliations

  1. Department of Micro-Nano Systems Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, 464-8603, Japan

    Fumihito Arai & Hisataka Maruyama

Authors
  1. Fumihito Arai
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  2. Hisataka Maruyama
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Correspondence to Fumihito Arai .

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Editors and Affiliations

  1. , Dept. of Mechanical and Industrial Eng., Northeastern University, Huntington Avenue 360, Boston, 02115, USA

    Constantinos Mavroidis

  2. ENSI Bourges, Institut PRISME, 88 Boulevard Lahitolle, Bourges, 18000, France

    Antoine Ferreira

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Arai, F., Maruyama, H. (2013). Nanorobotic Manipulation and Sensing for Biomedical Applications. In: Mavroidis, C., Ferreira, A. (eds) Nanorobotics. Springer, New York, NY. https://doi.org/10.1007/978-1-4614-2119-1_10

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  • DOI: https://doi.org/10.1007/978-1-4614-2119-1_10

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  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-1-4614-2118-4

  • Online ISBN: 978-1-4614-2119-1

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