Erythropoietin Detection: A Biosensor Approach
Erythropoietin is a glycoproteic hormone of 165 aminoacids belonging to hemopoietic growth factors. Recombinant EPO has been introduced in early ’80 to treat patients with severe anemia and to reduce side effects other EPO produced in eukaryotes have been introduced and different analogs produced over years. Since 2004, when EPO alfa and beta patents were over other molecules have appeared as EPO derivatives. EPO results in the prohibited list of World Anti-doping organization (WADA) and their abuse should be controlled in sport. EPO analysis is quite difficult since the molecule has relatively short half-life, numerous isoforms and many analogs are present on the market. We will report about EPO detection in urine samples. To achieve this, antibodies were used as recognition elements in sensing developments using Surface Plasmon Resonance (SPR) as transduction principle.
KeywordsSurface Plasmon Resonance Human Serum Albumin Reduce Side Effect Hemopoietic Growth Factor Transduction Principle
Maria Minunni would like to thank World Antidoping Agengy (WADA) for financial support, within the “Scientific Research Grant −2010 “Detection of Hepcidin as a new Biomarker of Erythropoiesis Stimulators Abuse: A Pilot Study”.
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