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Erythropoietin Detection: A Biosensor Approach

  • S. Scarano
  • M. L. Ermini
  • S. Tombelli
  • M. Mascini
  • M. Minunni
Conference paper
Part of the Lecture Notes in Electrical Engineering book series (LNEE, volume 109)

Abstract

Erythropoietin is a glycoproteic hormone of 165 aminoacids belonging to hemopoietic growth factors. Recombinant EPO has been introduced in early ’80 to treat patients with severe anemia and to reduce side effects other EPO produced in eukaryotes have been introduced and different analogs produced over years. Since 2004, when EPO alfa and beta patents were over other molecules have appeared as EPO derivatives. EPO results in the prohibited list of World Anti-doping organization (WADA) and their abuse should be controlled in sport. EPO analysis is quite difficult since the molecule has relatively short half-life, numerous isoforms and many analogs are present on the market. We will report about EPO detection in urine samples. To achieve this, antibodies were used as recognition elements in sensing developments using Surface Plasmon Resonance (SPR) as transduction principle.

Keywords

Surface Plasmon Resonance Human Serum Albumin Reduce Side Effect Hemopoietic Growth Factor Transduction Principle 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

Maria Minunni would like to thank World Antidoping Agengy (WADA) for financial support, within the “Scientific Research Grant −2010 “Detection of Hepcidin as a new Biomarker of Erythropoiesis Stimulators Abuse: A Pilot Study”.

References

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • S. Scarano
    • 1
  • M. L. Ermini
    • 1
  • S. Tombelli
    • 1
  • M. Mascini
    • 1
  • M. Minunni
    • 1
  1. 1.Dipartimento di Chimica “Ugo Schiff”Università degli Studi di FirenzeSesto F.noItaly

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